| Field | Specification |
|---|---|
| Target | |
| Alternative names | DNA fragmentation factor subunit alpha; DNA fragmentation factor 45 kDa subunit; DFF-45; Inhibitor of CAD; ICAD; DFFA; DFF1; DFF45 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human DFFA/ICAD recombinant protein (Position: E14-S308). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets DNA fragmentation factor subunit alpha (gene DFFA) in human and rat samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 36.5 kDa, and the supplier reports an observed band at about 45 kDa.
DFFA (DFF45, also called ICAD) is the inhibitor and chaperone of the DNase DFF40/CAD. In healthy cells it keeps the nuclease inactive; during apoptosis, caspase-3 cleaves DFFA, freeing DFF40 to fragment chromosomal DNA. DFFA is studied as part of the apoptotic DNA fragmentation pathway.
| Target | DNA fragmentation factor subunit alpha (gene DFFA; UniProt O00273, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.005 mg NaN3 |
| Calculated MW | 36.5 kDa |
| Observed MW | 45 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Rat) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HeLa cells, human HEK293 cells, human HepG2 cells, rat stomach tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): A431 cells.
Immunogen
Recombinant human DFFA/ICAD fragment (Glu14–Ser308), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places DNA fragmentation factor subunit alpha in the cytoplasm. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
