| Field | Specification |
|---|---|
| Target | |
| Alternative names | Microprocessor complex subunit DGCR8;DiGeorge syndrome critical region 8;DGCR8;C22orf12, DGCRK6;LP4941; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human DGCR8 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone EAO-4 is a rabbit monoclonal antibody that recognizes microprocessor complex subunit DGCR8 in human, mouse and rat samples and is validated for Western blot, ICC/IF and immunoprecipitation. The predicted molecular weight is 86.0 kDa, and the supplier reports an observed band at about 100 kDa.
DGCR8 is the RNA-binding partner of the RNase III enzyme Drosha in the Microprocessor complex. It recognizes primary microRNA transcripts in the nucleus and positions Drosha to cut out precursor microRNAs, the first step of canonical microRNA biogenesis. DGCR8 lies in the 22q11.2 deletion region and is studied in microRNA biology and neurodevelopment.
| Target | Microprocessor complex subunit DGCR8 (gene DGCR8; UniProt Q8WYQ5, human) |
|---|---|
| Clone | EAO-4 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 86.0 kDa |
| Observed MW | 100 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
Immunogen
Synthetic peptide based on the sequence of human DGCR8.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places microprocessor complex subunit DGCR8 in the nucleus. Tissue expression noted by UniProt (human): Ubiquitously expressed.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
