| Field | Specification |
|---|---|
| Target | |
| Alternative names | Acyl-CoA-binding protein;ACBP;Diazepam-binding inhibitor;DBI;Endozepine;EP;DBI; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human DBI recombinant protein (Position: S2-I87). Human DBI shares 77.9% amino acid (aa) sequence identity with both mouse and rat DBI. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects acyl-CoA-binding protein (gene DBI) in human samples and is validated for flow cytometry, ICC/IF, IHC and Western blot. The predicted molecular weight is 10.0 kDa, and the supplier reports an observed band at about 10 kDa.
Acyl-CoA-binding protein (ACBP, encoded by DBI) is a small, highly conserved protein that binds acyl-CoA esters of medium and long fatty acids with high affinity and helps shuttle them within the cell for lipid metabolism. It was originally named diazepam-binding inhibitor because it can displace benzodiazepines from GABAA receptors. It is studied in lipid metabolism, neurosteroid signaling and appetite regulation.
| Target | Acyl-CoA-binding protein (gene DBI; UniProt P07108, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 10.0 kDa |
| Observed MW | 10 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human) |
| IHC (frozen sections) | 0.5–1 µg/mL (Human) |
| Immunocytochemistry / Immunofluorescence | 2 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human COLO320 cells, human A549 cells, human PC-3 cells, human HepG2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human placenta tissue, human prostatic cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Samples with confirmed staining (ICC/IF): U2OS cells.
Samples with a confirmed signal (flow cytometry): MCF-7 cells, PC-3 cells, THP-1 cells.
Immunogen
Recombinant human DBI fragment (Ser2–Ile87), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 77.9%.
Reactivity Notes
The supplier lists reactivity with human. UniProt places acyl-CoA-binding protein in the endoplasmic reticulum and Golgi apparatus. Tissue expression noted by UniProt (human): Isoform 1 is ubiquitous, with a moderate expression level. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Kaiyang Chen et al. (2022) Oligosaccharide and short-chain fatty acid: A double-edged sword in obese mice by regulating food intake and fat synthesis. Food Research International. 10.1016/j.foodres.2022.111619
