| Field | Specification |
|---|---|
| Target | |
| Alternative names | Endoribonuclease Dicer;3.1.26.3;Helicase with RNase motif;Helicase MOI;DICER1;DICER, HERNA, KIAA0928; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human Dicer recombinant protein (Position: M1-N195). Human Dicer shares 94% amino acid (aa) sequence identity with mouse Dicer. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes endoribonuclease Dicer (gene DICER1) in human, mouse and rat samples and is validated for flow cytometry, IHC and Western blot. The predicted molecular weight is 218.7 kDa, and the supplier reports an observed band at about 250 kDa.
Dicer (DICER1) is an RNase III endoribonuclease that cuts long double-stranded RNAs and precursor microRNAs into short duplexes of about 21–23 nucleotides. These small RNAs are then loaded into Argonaute proteins to guide RNA silencing. Dicer is essential for microRNA production and is studied in gene regulation, development and DICER1 syndrome.
| Target | Endoribonuclease Dicer (gene DICER1; UniProt Q9UPY3, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 218.7 kDa |
| Observed MW | 250 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human HeLa cells, human K562 cells, human HepG2 cells, human 293T cells, rat C6 cells, mouse brain tissue, mouse NIH/3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human breast cancer tissue, human penis squamous cells carcinoma tissue, human cervical cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with a confirmed signal (flow cytometry): MCF-7 cells.
Immunogen
Recombinant human Dicer fragment (Met1–Asn195), expressed in E. coli. Sequence identity with the mouse ortholog: 94%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places endoribonuclease Dicer in the cytoplasm. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Gao Yu-en et al. (2016) Post-transcriptional modulation of protein phosphatase PPP2CA and tumor suppressor PTEN by endogenous siRNA cleaved from hairpin within PTEN mRNA 3′UTR in human liver cells. ACTA Pharmacologica Sinica. 10.1038/aps.2016.18
Yuen Gao et al. (2015) A hairpin within YAP mRNA 3′UTR functions in regulation at post-transcription level. Biochemical and Biophysical Research Communications. 10.1016/j.bbrc.2015.02.106
