| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Exosome complex exonuclease RRP44; Protein DIS3 homolog; Ribosomal RNA-processing protein 44; DIS3; KIAA1008; RRP44 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | E. coli-derived human DIS3 recombinant protein (Position: Q62-A304). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of DIS3 (Alkaline phosphatase, placental type) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-DIS3 Antibody catalog # A01736-1. Tested in ELISA, Flow Cytometry, IP, IF, ICC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E. coli-derived human DIS3 recombinant protein (Position: Q62-A304). (reported region: Q62-A304).
- Molecular weight context: reported MW: 109 kDa; calculated MW: 57954 MW
- Reactivity: Human,Rat
- Applications: ELISA, Flow Cytometry, IP, IF, ICC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Alkaline phosphatase, placental type; DIS3 homolog, exosome endoribonuclease and 3'-5' exoribonuclease. Exosome complex exonuclease RRP44 or Dis3 is an enzyme that in humans is encoded by the DIS3 gene. Its protein product is an RNase enzyme homologous to the yeast protein Rrp44, and can be part of the exosome complex in the nucleus of eukaryotic cells. By genomic sequence analysis, this gene is mapped to chromosome 13q21-q22. Expression of human DIS3 could partially complement Dis3 depletion in yeast. Functional note: Putative catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. DIS3 has both 3'-5' exonuclease and endonuclease activities. Reported localization: Nucleolus. Nucleoplasm. Nucleus. Cytoplasm. Expression/tissue context: Widely expressed.
Research relevance and current trends
- Cell Cycle: Researchers commonly examine how DIS3 (Alkaline phosphatase, placental type) relates to this theme using model systems and orthogonal readouts.
- Cell Division: Researchers commonly examine how DIS3 (Alkaline phosphatase, placental type) relates to this theme using model systems and orthogonal readouts.
- DNA/RNA: Researchers commonly examine how DIS3 (Alkaline phosphatase, placental type) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative DIS3 (Alkaline phosphatase, placental type) levels across conditions; band patterns may reflect isoforms and processing.
- IF/ICC: evaluate subcellular localization and co-localization patterns; signal can depend on fixation/permeabilization and epitope accessibility.
- Flow cytometry: quantify target-positive populations and shifts in expression; gating strategy and background staining controls are essential.
- ELISA-compatible use: when applicable, interpret signal as relative abundance across sample sets with consistent handling and dilution strategy.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
