| Field | Specification |
|---|---|
| Target | |
| Alternative names | Exosome complex exonuclease RRP44; Protein DIS3 homolog; Ribosomal RNA-processing protein 44; DIS3; KIAA1008; RRP44 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human DIS3 recombinant protein (Position: Q62-A304). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes exosome complex exonuclease RRP44 (gene DIS3) in human and rat samples and is validated for ELISA, flow cytometry, immunoprecipitation, ICC/IF and Western blot. The predicted molecular weight is 109.0 kDa, and the supplier reports an observed band at about 109 kDa.
DIS3 (also called RRP44) is a catalytic subunit of the nuclear RNA exosome, with both 3′-to-5′ exoribonuclease and endonuclease activities. The exosome trims and degrades many RNAs, including rRNA precursors, snoRNAs, unstable transcripts and faulty mRNAs. DIS3 is frequently mutated in multiple myeloma and is studied in RNA surveillance and processing.
| Target | Exosome complex exonuclease RRP44 (gene DIS3; UniProt Q9Y2L1, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 109.0 kDa |
| Observed MW | 109 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Rat) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Immunoprecipitation | 0.5–2 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HeLa cells, human Raji cells, human MCF-7 cells, rat testis tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): HeLa cells.
Samples with confirmed staining (ICC/IF): human breast cancer tissue.
Samples with a confirmed signal (flow cytometry): MCF-7 cells.
Samples with a confirmed result (IP): HeLa cells.
Immunogen
Recombinant human DIS3 fragment (Gln62–Ala304), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places exosome complex exonuclease RRP44 in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): Widely expressed. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
