| Field | Specification |
|---|---|
| Target | |
| Alternative names | Dickkopf-related protein 1; Dickkopf-1; Dkk-1; hDkk-1; SK; DKK1; UNQ492/PRO1008 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived mouse Dkk1 recombinant protein (Position: N73-H272). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets dickkopf-related protein 1 (gene Dkk1) in mouse and rat samples and is validated for IHC and ELISA. The predicted molecular weight is 29.3 kDa.
Dickkopf-1 (DKK1) is a secreted inhibitor of canonical Wnt signaling. It binds the Wnt co-receptors LRP5/6 and, together with Kremen proteins, promotes their removal from the cell surface. DKK1 is studied in embryonic head and limb development, bone formation and cancer, where it is often used as a Wnt pathway readout.
| Target | Dickkopf-related protein 1 (gene Dkk1; UniProt O54908, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 29.3 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 2–5 µg/mL (Mouse, Rat) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with confirmed staining (IHC): mouse brain tissue, mouse spleen tissue, rat brain tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant mouse Dkk1 fragment (Asn73–His272), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt describes dickkopf-related protein 1 as a secreted protein.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
