| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Dickkopf-related protein 2;Dickkopf-2;Dkk-2;hDkk-2;DKK2;UNQ682/PRO1316; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human DKK2 recombinant protein (Position: K34-I259). Human DKK2 shares 96% amino acid (aa) sequence identity with mouse DKK2. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of DKK2 (Dickkopf-related protein 2) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-DKK2 Antibody Picoband® catalog # PB9551. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E.coli-derived human DKK2 recombinant protein (Position: K34-I259). Human DKK2 shares 96% amino acid (aa) sequence identity with mouse DKK2. (reported region: K34-I259).
- Molecular weight context: reported MW: 28 kDa; calculated MW: 28447 MW
- Reactivity: Human,Rat
- Applications: WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Dickkopf-related protein 2; Dickkopf-related protein 2. Dickkopf-related protein 2 is a protein that in humans is encoded by the DKK2 gene. This gene encodes a protein that is a member of the dickkopf family. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. It can act as either an agonist or antagonist of Wnt/beta-catenin signaling, depending on the cellular context and the presence of the co-factor kremen 2. Activity of this protein is also modulated by binding to the Wnt co-receptor LDL-receptor related protein 6 (LRP6). Functional note: Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero- posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease (By similarity). . Reported localization: Secreted. Expression/tissue context: Expressed in heart, brain, skeletal muscle and lung.
Research relevance and current trends
- Secreted: Researchers commonly examine how DKK2 (Dickkopf-related protein 2) relates to this theme using model systems and orthogonal readouts.
- Signaling Pathways: Researchers commonly examine how DKK2 (Dickkopf-related protein 2) relates to this theme using model systems and orthogonal readouts.
- Stem Cells: Researchers commonly examine how DKK2 (Dickkopf-related protein 2) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative DKK2 (Dickkopf-related protein 2) levels across conditions; band patterns may reflect isoforms and processing.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Family / similarity context: Belongs to the DAXX family.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
