| Field | Specification |
|---|---|
| Target | |
| Alternative names | Dickkopf-related protein 2;Dickkopf-2;Dkk-2;hDkk-2;DKK2;UNQ682/PRO1316; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human DKK2 recombinant protein (Position: K34-I259). Human DKK2 shares 96% amino acid (aa) sequence identity with mouse DKK2. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets dickkopf-related protein 2 (gene DKK2) in human and rat samples and is validated for Western blot. The predicted molecular weight is 28.4 kDa, and the supplier reports an observed band at about 28 kDa.
Dickkopf-2 (DKK2) is a secreted member of the Dickkopf family that binds LRP5/6 and usually inhibits canonical Wnt signaling, although it can activate the pathway in some contexts. It has been linked to osteoblast differentiation, angiogenesis and tumor immunity. DKK2 is studied in Wnt signaling, bone biology and cancer.
| Target | Dickkopf-related protein 2 (gene DKK2; UniProt Q9UBU2, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 28.4 kDa |
| Observed MW | 28 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Rat) |
|---|
Samples with a confirmed band (WB): rat cardiac muscle tissue, rat brain tissue, A549 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human DKK2 fragment (Lys34–Ile259), expressed in E. coli. Sequence identity with the mouse ortholog: 96%.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt describes dickkopf-related protein 2 as a secreted protein. Tissue expression noted by UniProt (human): Expressed in heart, brain, skeletal muscle and lung. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
