| Field | Specification |
|---|---|
| Target | |
| Alternative names | Dihydrolipoyl dehydrogenase, mitochondrial; Dihydrolipoamide dehydrogenase; Glycine cleavage system L protein; DLD; GCSL; LAD; PHE3 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human DLDH |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 24D83 is a rabbit monoclonal antibody that recognizes dihydrolipoyl dehydrogenase, mitochondrial (gene DLD) in human, mouse and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 54.2 kDa, and the supplier reports an observed band at about 56 kDa.
Dihydrolipoamide dehydrogenase (DLD) is a flavoprotein that serves as the shared E3 subunit of the pyruvate, 2-oxoglutarate and branched-chain α-keto acid dehydrogenase complexes, and it is also part of the glycine cleavage system. It reoxidizes the lipoamide arms of these complexes, passing electrons to NAD+. DLD is studied in mitochondrial energy metabolism and inherited metabolic disease.
| Target | Dihydrolipoyl dehydrogenase, mitochondrial (gene DLD; UniProt P09622, human) |
|---|---|
| Clone | 24D83 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 54.2 kDa |
| Observed MW | 56 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Immunogen
Synthetic peptide based on the sequence of human DLDH.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places dihydrolipoyl dehydrogenase, mitochondrial in the mitochondrion matrix, nucleus and cell projection.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
