| Field | Specification |
|---|---|
| Target | |
| Alternative names | Doublesex- and mab-3-related transcription factor 1; DM domain expressed in testis protein 1; DMRT1; DMT1 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human DMRT1. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AFCA-4 is a rabbit monoclonal antibody that recognizes doublesex- and mab-3-related transcription factor 1 (gene DMRT1) in human samples and is validated for Western blot and immunoprecipitation. The predicted molecular weight is 39.5 kDa, and the supplier reports an observed band at about 34 kDa.
DMRT1 is a transcription factor with a DM DNA-binding domain that is central to male sex determination and testis development. It maintains Sertoli cell identity and controls the switch between mitosis and meiosis in spermatogonia. DMRT1 is conserved across vertebrates and is studied in sex differentiation, spermatogenesis and germ cell tumors.
| Target | Doublesex- and mab-3-related transcription factor 1 (gene DMRT1; UniProt Q9Y5R6, human) |
|---|---|
| Clone | AFCA-4 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 39.5 kDa |
| Observed MW | 34 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunoprecipitation | 1:80 |
Samples with a confirmed band (WB): HL-60 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human DMRT1.
Reactivity Notes
The supplier lists reactivity with human. UniProt places doublesex- and mab-3-related transcription factor 1 in the nucleus. Tissue expression noted by UniProt (human): Testis-specific. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
