| Field | Specification |
|---|---|
| Target | |
| Alternative names | DNA ligase 4; DNA ligase IV; Polydeoxyribonucleotide synthase [ATP] 4; LIG4 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human DNA Ligase IV |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ADHG-12 is a rabbit monoclonal antibody that recognizes DNA ligase 4 (gene LIG4) in human samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 104.0 kDa, and the supplier reports an observed band at about 103 kDa.
DNA ligase 4 (LIG4) catalyzes the final joining step of non-homologous end joining, the main pathway for repairing DNA double-strand breaks in mammalian cells. It works in a complex with XRCC4 and is essential for V(D)J recombination in developing lymphocytes. LIG4 deficiency causes immunodeficiency and radiosensitivity, and the enzyme is studied in DNA repair and genome editing.
| Target | DNA ligase 4 (gene LIG4; UniProt P49917, human) |
|---|---|
| Clone | ADHG-12 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 104.0 kDa |
| Observed MW | 103 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Immunogen
Synthetic peptide based on the sequence of human DNA Ligase IV.
Reactivity Notes
The supplier lists reactivity with human. UniProt places DNA ligase 4 in the nucleus. Tissue expression noted by UniProt (human): Testis, thymus, prostate and heart.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
