| Field | Specification |
|---|---|
| Target | |
| Alternative names | DNA polymerase beta; 5'-deoxyribose-phosphate lyase; 5'-dRP lyase; AP lyase; POLB |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human DNA Polymerase beta Repair polymerase. Conducts "gap-filling" DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. Has a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ACGH-16 is a rabbit monoclonal antibody that recognizes DNA polymerase beta (gene POLB) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and immunoprecipitation. The predicted molecular weight is 38.2 kDa, and the supplier reports an observed band at about 38 kDa.
DNA polymerase β (POLB) is the main gap-filling polymerase in base excision repair. After a damaged base is removed and the backbone is cut, it removes the leftover 5′-deoxyribose phosphate and inserts a single nucleotide so the strand can be sealed. POLB is widely studied in DNA damage responses and genome stability.
| Target | DNA polymerase beta (gene POLB; UniProt P06746, human) |
|---|---|
| Clone | ACGH-16 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 38.2 kDa |
| Observed MW | 38 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
Immunogen
Synthetic peptide based on the sequence of human DNA Polymerase beta Repair polymerase.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places DNA polymerase beta in the nucleus and cytoplasm.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
