| Field | Specification |
|---|---|
| Target | |
| Alternative names | DNA polymerase lambda ; Pol Lambda |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human DNA Polymerase lambda recombinant protein (Position: K307-N407). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects DNA polymerase lambda (gene POLL) in human, mouse and rat samples and is validated for ELISA, flow cytometry and Western blot. The predicted molecular weight is 63.5 kDa, and the supplier reports an observed band at about 63 kDa.
DNA polymerase λ (POLL) is an X-family DNA polymerase involved in several repair pathways. It fills small gaps during base excision repair, has 5′-deoxyribose phosphate lyase activity, and helps process DNA ends in non-homologous end joining of double-strand breaks. It can also add nucleotides without a template.
| Target | DNA polymerase lambda (gene POLL; UniProt Q9UGP5, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 63.5 kDa |
| Observed MW | 63 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HeLa cells, human MCF-7 cells, rat testis tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with a confirmed signal (flow cytometry): U2OS cells.
Immunogen
Recombinant human DNA Polymerase lambda fragment (Lys307–Asn407), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places DNA polymerase lambda in the nucleus and chromosome. Tissue expression noted by UniProt (human): Expressed in a number of tissues. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
