| Field | Specification |
|---|---|
| Target | |
| Alternative names | DNA (cytosine-5)-methyltransferase 1; Dnmt1; CXXC-type zinc finger protein 9; DNA methyltransferase HsaI; DNA MTase HsaI; M.HsaI; MCMT; DNMT1; AIM; CXXC9; DNMT |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human Dnmt1 recombinant protein (Position: D22-N126). |
| Molecular weight | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects DNA (gene DNMT1) in human samples and is validated for ELISA, flow cytometry, ICC/IF, IHC and Western blot. The predicted molecular weight is 183.2 kDa, and the supplier reports an observed band at about 200 kDa.
DNMT1 is the maintenance DNA methyltransferase that copies CpG methylation patterns onto newly synthesized DNA strands. With its partner UHRF1, it recognizes hemimethylated DNA at replication forks, allowing methylation marks to be inherited through cell division. DNMT1 is essential for development and is studied in epigenetics, genomic imprinting and cancer.
| Target | DNA (gene DNMT1; UniProt P26358, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 183.2 kDa |
| Observed MW | 200 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HeLa cells, human 293T cells, human SH-SY5Y cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 8% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human breast cancer tissue, human pancrease cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): U2OS cells.
Samples with a confirmed signal (flow cytometry): 293T cells.
Immunogen
Recombinant human Dnmt1 fragment (Asp22–Asn126), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places DNA in the nucleus and chromosome. Tissue expression noted by UniProt (human): Ubiquitous. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Huichao Zhao et al. (2022) Folic acid intervention changes liver Foxp3 methylation and ameliorates the damage caused by Th17/Treg imbalance after long-term alcohol exposure. Food & Function. 10.1039/D1FO04267J
Fu Yuxin et al. (2025) A Hybrid compound H93 treats prostate cancer by directly binding UHRF1 and promoting protein dimerization. Communications Chemistry. 10.1038/s42004-025-01744-3
Zhang Daoyu et al. (2020) Thioguanine Induces Apoptosis in Triple-Negative Breast Cancer by Regulating PI3K–AKT Pathway. Frontiers in Oncology. 10.3389/fonc.2020.524922
Er-Bao Bian et al. (2012) DNMT1-mediated PTEN hypermethylation confers hepatic stellate cell activation and liver fibrogenesis in rats. Toxicology and Applied Pharmacology. 10.1016/j.taap.2012.06.022
Zeng Peng et al. (2018) Emodin Rescued Hyperhomocysteinemia-Induced Dementia and Alzheimer’s Disease-Like Features in Rats. International Journal of Neuropsychopharmacology. 10.1093/ijnp/pyy090
Karine Assis Costa et al. (2024) Supplementation of l-arginine in pregnant gilts affects the protein abundance of DNMT1 in 35-day fetuses. Animal Reproduction Science. 10.1016/j.anireprosci.2024.107574
Humberto J. Ferreira et al. (2018) Circular RNA CpG island hypermethylation-associated silencing in human cancer. Oncotarget. 10.18632/oncotarget.25673
