| Field | Specification |
|---|---|
| Target | |
| Alternative names | Docking protein 1; Downstream of tyrosine kinase 1; p62(dok); pp62; DOK1 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human DOK1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 25D44 is a rabbit monoclonal antibody that recognizes docking protein 1 (gene DOK1) in human, mouse and rat samples and is validated for Western blot and ICC/IF. The predicted molecular weight is 52.4 kDa, and the supplier reports an observed band at about 65 kDa.
DOK1 (downstream of tyrosine kinase 1) is an adaptor protein without enzymatic activity that is phosphorylated by many tyrosine kinases. Once phosphorylated, it provides docking sites for signaling proteins such as RasGAP and generally dampens Ras-MAPK signaling. DOK1 also modulates integrin activation and is studied in immune signaling and tumor suppression.
| Target | Docking protein 1 (gene DOK1; UniProt Q99704, human) |
|---|---|
| Clone | 25D44 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 52.4 kDa |
| Observed MW | 65 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): K562 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human DOK1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. Tissue expression noted by UniProt (human): Expressed in pancreas, heart, leukocyte and spleen. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
