| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Tumor necrosis factor receptor superfamily member 25;Apo-3;Apoptosis-inducing receptor AIR;Apoptosis-mediating receptor DR3;Apoptosis-mediating receptor TRAMP;Death receptor 3;Lymphocyte-associated receptor of death;LARD;Protein WSL;Protein WSL-1;TNFRSF25;APO3, DDR3, DR3, TNFRSF12, WSL, WSL1;UNQ455/PRO779; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human DR3. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-DR3/TNFRSF25 Antibody Picoband® is an antibody targeting TNFRSF25. Common applications include WB, IHC, Flow Cytometry, ELISA. Key specifications include host: Rabbit; clonality: Polyclonal; isotype: Rabbit IgG; reactivity: Human; observed MW: 59 kDa; calculated MW: 45385 MW.
Boster Bio Anti-DR3/TNFRSF25 Antibody catalog # PA2004. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Target: TNFRSF25 — Tumor necrosis factor receptor superfamily member 25
- Antibody format: Host: Rabbit; Clonality: Polyclonal; Isotype: Rabbit IgG
- Species reactivity: Human
- Molecular weight guidance: Observed: 59 kDa; Calculated: 45385 MW
Specificity note: No cross reactivity with other proteins.
Biological background
Protein function (datasheet): Receptor for TNFSF12/APO3L/TWEAK. Interacts ly with the adapter TRADD. Mediates activation of NF-kappa-B and induces apoptosis. May play a role in regulating lymphocyte homeostasis.
Scientific background (datasheet): TNFRSF25 (Tumor Necrosis Factor Receptor Superfamily Member 25), also known as LARD, APO3, DR3 or TNFR25, is a protein that in humans is encoded by the TNFRSF25 gene. Members of the mammalian tumor necrosis factor receptor (TNFR) family are cell-surface proteins that interact with a corresponding TNF-related ligand family. By fluorescence in situ hybridization, Marsters et al. (1996) mapped the Apo3 gene to 1p36.3. Marsters et al. (1996) showed that ectopic expression of Apo3 in mammalian cells triggered apoptosis and activated the transcription factor NF-kappa-B. They suggested that, like TNFR1, Apo3 may regulate distinct signaling pathways in different cellular contexts.
Cellular localization (datasheet): Isoform 1: Cell membrane; Single-pass type I membrane protein.
Tissue details (datasheet): Abundantly expressed in thymocytes and lymphocytes. Detected in lymphocyte-rich tissues such as thymus, colon, intestine, and spleen. Also found in the prostate.
Sequence similarities (datasheet): Contains 1 death domain.
Research relevance and current trends
- Commonly studied in contexts related to Signaling Pathway.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- ELISA: Measure target abundance in compatible matrices using a standard-curve readout; ensure dilution linearity and appropriate controls.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
- Flow cytometry: Quantify target-positive populations in single-cell suspensions; pair with viability and isotype/FMO controls conceptually.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Cross-reactivity (datasheet): No cross-reactivity with other proteins
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a polyclonal antibody, this reagent may recognize multiple epitopes on the target, which can improve detection robustness but may require careful specificity controls.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
