| Field | Specification |
|---|---|
| Target | |
| Alternative names | Tumor necrosis factor receptor superfamily member 10A;Death receptor 4;TNF-related apoptosis-inducing ligand receptor 1;TRAIL receptor 1;TRAIL-R1;CD261;TNFRSF10A;APO2, DR4, TRAILR1; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human DR4. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects tumor necrosis factor receptor superfamily member 10A (gene TNFRSF10A) in human, mouse and rat samples and is validated for ICC/IF and Western blot. The predicted molecular weight is 50.1 kDa, and the supplier reports an observed band at about 45 kDa.
TRAIL-R1 (TNFRSF10A, DR4) is a death receptor for the cytokine TRAIL. Ligand binding recruits FADD and caspase-8 to form the death-inducing signaling complex, which starts the caspase cascade of apoptosis. TRAIL-R1 is studied in apoptosis, immune surveillance of tumors and TRAIL-based cancer research.
| Target | Tumor necrosis factor receptor superfamily member 10A (gene TNFRSF10A; UniProt O00220, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.01 mg NaN3 |
| Calculated MW | 50.1 kDa |
| Observed MW | 45 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL |
Samples with a confirmed band (WB): human A549 cells, human Hacat cells, human RT4 cells, human K562 cells, rat spleen tissue, rat lung tissue, mouse spleen tissue, mouse lung tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): U2OS cells.
Immunogen
Synthetic peptide from the N-terminal region of human DR4.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places tumor necrosis factor receptor superfamily member 10A in the cell membrane, membrane raft and cytoplasm. Tissue expression noted by UniProt (human): Widely expressed. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Fu K et al. (2011) DJ-1 inhibits TRAIL-induced apoptosis by blocking pro-caspase-8 recruitment to FADD. Oncogene. 10.1038/onc.2011.315
Nianli Liu et al. (2014) miR-942 decreases TRAIL-induced apoptosis through ISG12a downregulation and is regulated by AKT. Oncotarget. 10.18632/oncotarget.2067
Peiyuan Jia et al. (2013) The Novel Fusion Proteins, GnRH-p53 and GnRHIII-p53, Expression and Their Anti-Tumor Effect. PLoS One. 10.1371/journal.pone.0079384
Zhang Liang et al. (2008) The Occurrence and Regional Distribution of DR4 on Herniated Disc Cells: A Potential Apoptosis Pathway in Lumbar Intervertebral Disc. Spine. 10.1097/BRS.0b013e318163e036
Li Jiawen et al. (2010) Plumbagin enhances TRAIL-mediated apoptosis through up-regulation of death receptor in human melanoma A375 cells. Journal of Huazhong University of Science and Technology-Medical Sciences. 10.1007/s11596-010-0449-x
