| Field | Specification |
|---|---|
| Target | |
| Alternative names | Dysferlin;Dystrophy-associated fer-1-like protein;Fer-1-like protein 1;DYSF;FER1L1; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Dysferlin |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone EGO-4 is a rabbit monoclonal antibody that recognizes dysferlin in human and mouse samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 237.3 kDa, and the supplier reports an observed band at about 280 kDa.
Dysferlin (DYSF) is a large membrane protein with multiple Ca2+-binding C2 domains. In skeletal and heart muscle it helps reseal the plasma membrane after mechanical damage, and its loss causes limb-girdle muscular dystrophy type 2B and Miyoshi myopathy.
| Target | Dysferlin (gene DYSF; UniProt O75923, human) |
|---|---|
| Clone | EGO-4 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 237.3 kDa |
| Observed MW | 280 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): mouse skeletal muscle tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human dysferlin.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt places dysferlin in the cell membrane, cytoplasmic vesicle membrane and late endosome membrane. Tissue expression noted by UniProt (human): Expressed in skeletal muscle, myoblast, myotube and in the syncytiotrophoblast (STB) of the placenta (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
