Anti-EAAT3/SLC1A1 Antibody Picoband®

Overview

Anti-EAAT3/SLC1A1 Antibody Picoband® is an antibody for SLC1A1 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.

Key elements and design rationale

• Target: SLC1A1 (Neuronal acetylcholine receptor subunit alpha-5); UniProt: P43005
• Antibody format: Rabbit, Polyclonal, Rabbit IgG
• Molecular weight: 70 kDa, calculated 53054 MW
• Applications: WB, IHC, Flow Cytometry, ELISA

Vendor description (summary): Boster Bio Anti-EAAT3/SLC1A1 Antibody Picoband® catalog # A02367-1.

Biological background

Biological context: After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.

Expression and localization notes: cellular localization: Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein..

Common research applications

• Western blotting (WB): Compare SLC1A1 levels across samples and conditions using appropriate loading and biological controls.
• Immunohistochemistry (IHC): Evaluate spatial distribution of SLC1A1 in tissue sections, considering fixation and antigen retrieval effects.
• Flow cytometry: Quantify SLC1A1-positive populations in single-cell suspensions with appropriate gating and controls.
• ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.

Notes for experimental interpretation

• Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
• Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.

Additional product notes (from provided fields)

• Background: Solute carrier family 1 member 1, also called SLC1A1, is a protein that in humans is encoded by the SLC1A1 gene. By Southern analysis of a panel of human/rodent somatic cell hybrids and by fluorescence in situ hybridization (FISH), this gene is mapped to 9p24.2. This gene encodes a member of the high-affinity glutamate transporters that play an essential role in transporting glutamate across plasma membranes. In brain, these transporters are crucial in terminating the postsynaptic action of the neurotransmitter glutamate, and in maintaining extracellular glutamate concentrations below neurotoxic levels. This transporter also transports aspartate, and mutations in this gene are though to cause dicarboxylicamino aciduria, also known as glutamate-aspartate transport defect.
• Cross reactivity: No cross-reactivity with other proteins
• Cellular localization: Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.
• Research category: Ligand-Gated Ion Channels,Neuroscience,Neurotransmission,Receptors / Channels

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.