| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Excitatory amino acid transporter 3;Excitatory amino-acid carrier 1;Sodium-dependent glutamate/aspartate transporter 3;Solute carrier family 1 member 1;Slc1a1;Eaac1, Eaat3; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of mouse EAAT3, identical to the related rat sequence. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-EAAT3/SLC1A1 Antibody Picoband® is an antibody targeting SLC1A1. Common applications include WB, IHC, Flow Cytometry, ELISA. Key specifications include host: Rabbit; clonality: Polyclonal; isotype: Rabbit IgG; reactivity: Rat,Mouse; observed MW: 15 kDa; calculated MW: 56694 MW.
Boster Bio Anti-EAAT3/SLC1A1 Antibody catalog # PA2164-2. Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Target: SLC1A1 — Excitatory amino acid transporter 3
- Antibody format: Host: Rabbit; Clonality: Polyclonal; Isotype: Rabbit IgG
- Species reactivity: Rat,Mouse
- Molecular weight guidance: Observed: 15 kDa; Calculated: 56694 MW
Specificity note: No cross reactivity with other proteins.
Biological background
Protein function (datasheet): Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium (By similarity). Negatively regulated by ARL6IP5 (PubMed:12119102). .
Scientific background (datasheet): Solute carrier family 1 member 1, also called SLC1A1, is a protein that in humans is encoded by the SLC1A1 gene. By Southern analysis of a panel of human/rodent somatic cell hybrids and by fluorescence in situ hybridization (FISH), this gene is mapped to 9p24.2. This gene encodes a member of the high-affinity glutamate transporters that play an essential role in transporting glutamate across plasma membranes. In brain, these transporters are crucial in terminating the postsynaptic action of the neurotransmitter glutamate, and in maintaining extracellular glutamate concentrations below neurotoxic levels. This transporter also transports aspartate, and mutations in this gene are though to cause dicarboxylicamino aciduria, also known as glutamate-aspartate transport defect.
Cellular localization (datasheet): Cell membrane ; Multi-pass membrane protein. Apical cell membrane ; Multi-pass membrane protein. Membrane; Multi-pass membrane protein.
Tissue details (datasheet): Expressed in brain, lung, kidney and skeletal muscle.
Research relevance and current trends
- Commonly studied in contexts related to Amino Acids,Neuroscience,Neurotransmitter,Transporters.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- ELISA: Measure target abundance in compatible matrices using a standard-curve readout; ensure dilution linearity and appropriate controls.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
- Flow cytometry: Quantify target-positive populations in single-cell suspensions; pair with viability and isotype/FMO controls conceptually.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Cross-reactivity (datasheet): No cross-reactivity with other proteins
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a polyclonal antibody, this reagent may recognize multiple epitopes on the target, which can improve detection robustness but may require careful specificity controls.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
