| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Na (+)/H (+) exchange regulatory cofactor NHE-RF1; NHERF-1; Ezrin-radixin-moesin-binding phosphoprotein 50; EBP50; Regulatory cofactor of Na (+)/H (+) exchanger; Sodium-hydrogen exchanger regulatory factor 1; Solute carrier family 9 isoform A3 regulatory factor 1; SLC9A3R1; NHERF; NHERF1 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human EBP50/NHERF-1/SLC9A3R1 recombinant protein (Position: K69-R338). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-EBP50/NHERF-1/SLC9A3R1 Antibody Picoband® is an antibody for SLC9A3R1 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: SLC9A3R1 (SLC9A3 regulator 1); UniProt: O14745
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 50 kDa, calculated 38779 MW
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-EBP50/NHERF-1/SLC9A3R1 Antibody Picoband® catalog # A02427-1.
Biological background
Biological context: Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for recycling of internalized ADRB2. Was first known to play a role in the regulation of the activity and subcellular location of SLC9A3. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May enhance Wnt signaling. May participate in HTR4 targeting to microvilli. Involved in the regulation of phosphate reabsorption in the renal proximal tubules. Involved in sperm capacitation. May participate in the regulation of the chloride and bicarbonate homeostasis in spermatozoa.
Expression and localization notes: cellular localization: Apical cell membrane. Cytoplasm. Endomembrane system. Peripheral membrane protein. Filopodium. Ruffle. Microvillus., tissue context: Detected in liver, kidney, pancreas, prostate, spleen, small intestine and placenta, in particular in the syncytiotrophoblast..
Common research applications
- Western blotting (WB): Compare SLC9A3R1 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of SLC9A3R1 in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify SLC9A3R1-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Specificity: No cross reactivity with other proteins.
- Background: Sodium-hydrogen antiporter 3 regulator 1 is a regulator of Sodium-hydrogen antiporter 3. It is encoded by the gene SLC9A3R1. It is mapped to 17q25.1. This gene encodes a sodium/hydrogen exchanger regulatory cofactor. The protein interacts with and regulates various proteins including the cystic fibrosis transmembrane conductance regulator and G-protein coupled receptors such as the beta2-adrenergic receptor and the parathyroid hormone 1 receptor. The protein also interacts with proteins that function as linkers between integral membrane and cytoskeletal proteins. The protein localizes to actin-rich structures including membrane ruffles, microvilli, and filopodia. Mutations in this gene result in hypophosphatemic nephrolithiasis/osteoporosis type 2, and loss of heterozygosity of this gene is implicated in breast cancer.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Apical cell membrane. Cytoplasm. Endomembrane system. Peripheral membrane protein. Filopodium. Ruffle. Microvillus.
- Tissue details: Detected in liver, kidney, pancreas, prostate, spleen, small intestine and placenta, in particular in the syncytiotrophoblast.
- Research category: Actin Binding Proteins,Actin, etc.,Adapters,Cancer,Cell Type Markers,Cytoskeleton,Cytoskeleton/ECM,Extracellular Matrix,Invasion/Microenvironment,Microfilaments,Signal Transduction,Tags & Cell Markers,Transmembrane
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
