| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Na (+)/H (+) exchange regulatory cofactor NHE-RF1;NHERF-1;Ezrin-radixin-moesin-binding phosphoprotein 50;EBP50;Regulatory cofactor of Na (+)/H (+) exchanger;Sodium-hydrogen exchanger regulatory factor 1;Solute carrier family 9 isoform A3 regulatory factor 1;SLC9A3R1;NHERF, NHERF1; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthesized peptide derived from human EBP50 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-EBP50 SLC9A3R1 Rabbit Monoclonal Antibody is an antibody targeting SLC9A3R1. Common applications include WB, IHC. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: IIH-19; isotype: Rabbit IgG; reactivity: Human,Rat; observed MW: 80 kDa; calculated MW: 38868 MW.
Boster Bio Anti-EBP50 SLC9A3R1 Rabbit Monoclonal Antibody catalog # M02427. Tested in WB, IHC applications. This antibody reacts with Human, Rat.
Key elements and design rationale
- Target: SLC9A3R1 — Na(+)/H(+) exchange regulatory cofactor NHE-RF1
- Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: IIH-19; Isotype: Rabbit IgG
- Species reactivity: Human,Rat
- Molecular weight guidance: Observed: 80 kDa; Calculated: 38868 MW
Biological background
Protein function (datasheet): Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for recycling of internalized ADRB2. Was first known to play a role in the regulation of the activity and subcellular location of SLC9A3. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May enhance Wnt signaling. May participate in HTR4 targeting to microvilli (By similarity). Involved in the regulation of phosphate reabsorption in the renal proximal tubules. Involved in sperm capacitation. May participate in the regulation of the chloride and bicarbonate homeostasis in spermatozoa. .
Cellular localization (datasheet): Cytoplasm . Apical cell membrane . Endomembrane system; Peripheral membrane protein. Cell projection, filopodium. Cell projection, ruffle. Cell projection, microvillus. Translocates from the cytoplasm to the apical cell membrane in a PODXL-dependent manner. Colocalizes with CFTR at the midpiece of sperm tail (By similarity). Colocalizes with actin in microvilli-rich apical regions of the syncytiotrophoblast. Found in microvilli, ruffling membrane and filopodia of HeLa cells. Present in lipid rafts of T- cells. .
Tissue details (datasheet): Detected in liver, kidney, pancreas, prostate, spleen, small intestine and placenta, in particular in the syncytiotrophoblast. .
Research relevance and current trends
- Commonly studied in contexts related to Actin Binding Proteins,Actin, etc.,Adapters,Cancer,Cell Type Markers,Cytoskeleton,Cytoskeleton/ECM,Extracellular Matrix,Invasion/Microenvironment,Microfilaments,Signal Transduction,Tags & Cell Markers,Transmembrane.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
