| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Ectodysplasin-A;Ectodermal dysplasia protein;EDA protein;Ectodysplasin-A, membrane form;Ectodysplasin-A, secreted form;EDA;ED1, EDA2; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human EDA recombinant protein (Position: A30-S391). Human EDA shares 95% amino acid (aa) sequence identity with mouse EDA. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of EDA (Ectodysplasin-A) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-EDA Antibody Picoband® catalog # PB9191. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E.coli-derived human EDA recombinant protein (Position: A30-S391). Human EDA shares 95% amino acid (aa) sequence identity with mouse EDA. (reported region: A30-S391).
- Molecular weight context: reported MW: 43 kDa; calculated MW: 41294 MW
- Reactivity: Human
- Applications: WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Ectodysplasin-A; Ectodysplasin-A. Ectodysplasin-A is a protein that in humans is encoded by the EDA gene. It is mapped to Xq13.1. The protein encoded by this gene is a type II membrane protein that can be cleaved by furin to produce a secreted form. The encoded protein, which belongs to the tumor necrosis factor family, acts as a homotrimer and may be involved in cell-cell signaling during the development of ectodermal organs. Defects in this gene are a cause of ectodermal dysplasia, anhidrotic, which is also known as X-linked hypohidrotic ectodermal dysplasia. Functional note: Seems to be involved in epithelial-mesenchymal signaling during morphogenesis of ectodermal organs. Isoform 1 binds only to the receptor EDAR, while isoform 3 binds exclusively to the receptor XEDAR. Reported localization: Cell membrane; Single-pass type II membrane protein. Expression/tissue context: Not abundant; expressed in specific cell types of ectodermal (but not mesodermal) origin of keratinocytes, hair follicles, sweat glands. Also in adult heart, liver, muscle, pancreas, prostate, fetal liver, uterus, small intestine and umbilical chord. .
Research relevance and current trends
- Developmental Biology: Researchers commonly examine how EDA (Ectodysplasin-A) relates to this theme using model systems and orthogonal readouts.
- Growth Factors/Hormones: Researchers commonly examine how EDA (Ectodysplasin-A) relates to this theme using model systems and orthogonal readouts.
- Lineage Markers: Researchers commonly examine how EDA (Ectodysplasin-A) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative EDA (Ectodysplasin-A) levels across conditions; band patterns may reflect isoforms and processing.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Family / similarity context: Belongs to the tumor necrosis factor family.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
