| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Early endosome antigen 1; Endosome-associated protein p162; Zinc finger FYVE domain-containing protein 2; EEA1; ZFYVE2 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human EEA1, identical to the related mouse and rat sequences. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of EEA1 (Arachidonate 12-lipoxygenase, 12S-type) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-EEA1 Antibody Picoband® catalog # A02296-3. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: A synthetic peptide corresponding to a sequence at the C-terminus of human EEA1, identical to the related mouse and rat sequences.
- Molecular weight context: reported MW: 170 kDa; calculated MW: 162466 MW
- Reactivity: Human,Mouse,Rat
- Applications: IF, IHC, ICC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Arachidonate 12-lipoxygenase, 12S-type; early endosome antigen 1. The gene EEA1 encodes for the 1400 amino acid protein, Early Endosome Antigen 1. It localizes exclusively to early endosomes and has an important role in endosomal trafficking. EEA1 binds ly to the phospholipid phosphatidylinositol 3-phosphate through its C-terminal FYVE domain and forms ahomodimer through a coiled coil. Furthermore, EEA1 acts as a tethering molecule that couples vesicle docking with SNAREs such as N-ethylmaleimide sensitive fusion protein, bringing the endosomes physically closer and ultimately resulting in the fusion and delivery of endosomal cargo. Functional note: Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking. Reported localization: Cytoplasm. Early endosome membrane; Peripheral membrane protein. Expression/tissue context: Expressed in heart, skeletal muscle, pancreas, kidney, liver, lung, placenta and brain. Also expressed in testis and thymus.
Research relevance and current trends
- Signal Transduction: Researchers commonly examine how EEA1 (Arachidonate 12-lipoxygenase, 12S-type) relates to this theme using model systems and orthogonal readouts.
- Subcellular Markers: Researchers commonly examine how EEA1 (Arachidonate 12-lipoxygenase, 12S-type) relates to this theme using model systems and orthogonal readouts.
- Tags & Cell Markers: Researchers commonly examine how EEA1 (Arachidonate 12-lipoxygenase, 12S-type) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative EEA1 (Arachidonate 12-lipoxygenase, 12S-type) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of EEA1 (Arachidonate 12-lipoxygenase, 12S-type) across tissue regions and cell types using matched controls.
- IF/ICC: evaluate subcellular localization and co-localization patterns; signal can depend on fixation/permeabilization and epitope accessibility.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
