| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Protein argonaute-1;Argonaute1;hAgo1;Argonaute RISC catalytic component 1;Eukaryotic translation initiation factor 2C 1;eIF-2C 1;eIF2C 1;Putative RNA-binding protein Q99;AGO1;EIF2C1; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human EEF2 recombinant protein (Position: M1-L858). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-EEF2 Antibody Picoband® is an antibody for EEF2 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Monkey,Mouse,Rat,Zebrafish. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: EEF2 (Protein argonaute-1); UniProt: P13639
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 95 kDa, calculated 97214 MW
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-EEF2 Antibody Picoband® catalog # A00830-1.
Biological background
Biological context: Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) or short interfering RNAs (siRNAs), and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Also required for transcriptional gene silencing (TGS) of promoter regions which are complementary to bound short antigene RNAs (agRNAs). .
Expression and localization notes: cellular localization: Cytoplasm, P-body ., tissue context: Detected in blood plasma (at protein level)..
Common research applications
- Western blotting (WB): Compare EEF2 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of EEF2 in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify EEF2-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: Eukaryotic elongation factor 2 is a protein that in humans is encoded by the EEF2 gene. This gene encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation.
- Cross reactivity: No cross-reactivity with other proteins
- Cellular localization: Cytoplasm, P-body .
- Tissue details: Detected in blood plasma (at protein level).
- Research category: Cell Biology,Cell Cycle,Cell Division,Cytokinesis,DNA/RNA,Epigenetics and Nuclear Signaling,Nuclear Receptors,Nuclear Signaling Pathways,RNA Processing,Translation
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
