| Field | Specification |
|---|---|
| Target | |
| Alternative names | Elongation factor 2; EF-2; EEF2; EF2 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human EEF2 recombinant protein (Position: M1-L858). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets elongation factor 2 (gene EEF2) in human, monkey, mouse, rat and zebrafish samples and is validated for Western blot, IHC and ELISA. The predicted molecular weight is 95.3 kDa, and the supplier reports an observed band at about 95 kDa.
Elongation factor 2 (EEF2) is a GTPase that moves the ribosome along mRNA after each peptide bond is formed during translation. Its activity is switched off by eEF2 kinase phosphorylation, and it is a well-known target of diphtheria toxin.
| Target | Elongation factor 2 (gene EEF2; UniProt P13639, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Monkey, Mouse, Rat, Zebrafish |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 95.3 kDa |
| Observed MW | 95 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.25 µg/mL (Human, Monkey, Mouse, Rat, Zebrafish) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human, Mouse, Rat) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human Jurkat cells, monkey COS-7 cells, human HeLa cells, human 293T cells, human HepG2 cells, human Daudi cells, human MCF-7 cells, zebrafish tissue, rat stomach tissue, rat pancrease tissue, rat C6 cells, mouse pancrease tissue, mouse 3T3L1 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.25 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human breast cancer tissue, human laryngeal squamous cells carcinoma tissue, human lymphoma tissue, human renal cancer tissue, mouse ovary tissue, rat ovary tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human EEF2 fragment (Met1–Leu858), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, monkey, mouse, rat and zebrafish. UniProt places elongation factor 2 in the cytoplasm and nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
