Anti-EGF Rabbit Monoclonal Antibody

Overview

Anti-EGF Rabbit Monoclonal Antibody is an antibody targeting EGF. Common applications include WB, IP. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: ABOF-5; isotype: Rabbit IgG; reactivity: Human; observed MW: 125 kDa; calculated MW: 133994 MW.

Boster Bio Anti-EGF Rabbit Monoclonal Antibody catalog # M00378. Tested in WB, IP applications. This antibody reacts with Human.

Key elements and design rationale

• Target: EGF — Pro-epidermal growth factor
• Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: ABOF-5; Isotype: Rabbit IgG
• Species reactivity: Human
• Molecular weight guidance: Observed: 125 kDa; Calculated: 133994 MW

Biological background

Protein function (datasheet): EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941). .

Cellular localization (datasheet): Membrane; Single-pass type I membrane protein.

Tissue details (datasheet): Expressed in kidney, salivary gland, cerebrum and prostate. .

Research relevance and current trends

• Commonly studied in contexts related to Cancer,Cell Biology,Cell Cycle,Cell Differentiation,Growth Factors,Growth Factors/Hormones,Signal Transduction.
• Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
• Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).

Common research applications

• Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.

Notes for experimental interpretation

• Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
• Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.

As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.