Anti-EGFR

SKU:BHA10700172
Overview
Click light‑blue chips for details
EGFR-specific rabbit monoclonal antibody. IHC-P validated at 1:100 in Human samples. In vitro cloning technology ensures high specificity.
Format Unconjugated
Application IHC-P Optimized
Technology In Vitro Cloned
Immunogen Peptide derived from C-terminal region of human epidermal growth factor receptor (EGFR)
Clone A20-E
Available Options
Size
Options selector
Catalog no. Format Volume
DB 092-0.1 Concentrated
DB 092-RTU-7 Ready To Use
Field Specification
Clonality
  • Monoclonal
Host Rabbit
Immunogen Peptide derived from C-terminal region of human epidermal growth factor receptor (EGFR)
Isotype
  • IgG
Product Type
  • Primary Antibody
Reactivity
  • Human
Target EGFR

Product Overview

IHC Primary Antibody

IHC-optimized antibody targeting EGFR. Designed for paraffin-embedded tissue sections (IHC-P). Validated for use at 1:100 dilution.

Validated Application

Application Recommended Dilution
Immunohistochemistry – Paraffin (IHC-P) 1:100
✔ IHC-Optimized | DB Biotech Inc

Antibody Specifications

Target EGFR
Host Rabbit
Clonality Monoclonal
Isotype IgG
Conjugate Unconjugated
Reactivity Human
Purification Affinity Purification
Immunogen Peptide derived from C-terminal region of human epidermal growth factor receptor (EGFR)
Clone A20-E
UniProt P00533
Stabilizer 20 mg/ml BSA
Preservative 0.05% NaN3
Formulation nan
Storage Buffer 20 mM Tris-HCl, pH 8.0
Storage nan

Safety & Handling

⚠ Research Use Only (RUO). Not for diagnostic or therapeutic use. Contains sodium azide. Handle with standard laboratory precautions.
TargetEGFR
HostRabbit
ClonalityMonoclonal
ConjugateUnconjugated
ReactivityHuman
ApplicationIHC-P
Dilution1:100
PurificationAffinity Purification
CloneA20-E
ImmunogenPeptide derived from C-terminal region of human epidermal growth factor receptor (EGFR)

Validated for IHC-P on paraffin-embedded sections.

ApplicationDilution
IHC-P (Paraffin)1:100

Immunogen: Peptide derived from C-terminal region of human epidermal growth factor receptor (EGFR)

Clone: A20-E

What tissue types has this antibody been optimized for?
This antibody has been optimized for immunohistochemistry on paraffin-embedded (IHC-P) tissue sections from Human samples. Performance in frozen sections or other formats may require additional optimization.
What is the recommended working dilution?
The validated starting dilution is 1:100 for IHC-P. Optimal dilution may vary depending on tissue fixation, antigen retrieval method, and detection system. Titration is recommended for each new tissue type.
How was this antibody produced?
This is a rabbit monoclonal antibody produced by in vitro cloning technology. The clone designation is A20-E. This production method ensures a monospecific product with consistent lot-to-lot performance — critical for reproducible IHC results across experiments.
What antigen retrieval method is recommended?
Heat-induced epitope retrieval (HIER) in citrate buffer (pH 6.0) or EDTA buffer (pH 9.0) is generally recommended for paraffin-embedded sections. The optimal retrieval method may vary by antibody and tissue type.
What storage conditions are required?
Store undiluted at 4°C. Do not freeze. This product is stable when stored correctly for up to 12 months.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

Research budgets are tight — we get it. That's why we've put together a fresh round of exclusive promotions designed to help you stock up on the reagents, kits, and consumables your lab depends on, without stretching your budget.

🔬 What's on offer right now:

10% Off Pre-Designed siRNA Sets

20% Off Transmembrane Proteins

$50 Off All ELISA Kits

50% Off Lab Consumables + Free Shipping

$99 Pipette Filler Promotion Package

BlasTaq 2X qPCR MasterMix - 50% OFF Limited Time Offer

DENARASE® Endonuclease — 10% Off One Order

10% OFF CELL LINES-Limited-Time Offer

15% Off Proteins from Trusted Suppliers — Limited Time

👉 Browse all current deals

Get a Quote

Please use this form for bulk quantity requests or customized products.

Contact Information

Product Information

Supplier Ads Slides show

Add dynamic ads with slider