Anti-EGFR (Phospho-Tyr1092) Antibody

SKU:BHA10918191
Suppliers
Applied Biological Materials (abm) Inc.
Applied Biological Materials (abm) Inc.
Details Products
Overview
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Clonality Polyclonal
Species Reactivity Human,Mouse,Rat
Validated Applications Western Blot, Immunohistochemistry
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Catalog no. Size
Y011081 100 ug
Field Specification
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using; epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed; by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Product Overview

Research Use Only

The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor.

Validated Applications

Western Blot (WB) 1:500~1:1000 IHC: 1:50~1:100
Immunohistochemistry (IHC) 1:50~1:100

Key Specifications

Clonality Polyclonal
Species Reactivity Human,Mouse,Rat
Concentration 1ug/ul
Formulation Liquid
Catalog No. Y011081
Alternative Names ERBB1

Immunogen / Antigen

The antiserum was produced against synthesized phosphopeptide derived from human EGFR around the phosphorylation site of tyrosine1092 (P-E-YP-I-N).

Reactivity Notes

EGFR (phospho-tyr1092) antibody detects endogenous levels of EGFR only when phosphorylated at tyrosine 1092.

Additional Information

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100

Storage & Handling

This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Safety

For Research Use Only (RUO). Not intended for diagnostic, therapeutic, or veterinary use. Handle according to standard laboratory biosafety guidelines. Avoid skin and eye contact. Dispose of waste in accordance with local regulations.

What applications has the Anti-EGFR (Phospho-Tyr1092) Antibody been validated for?

This antibody has been validated for: Western Blot, Immunohistochemistry. For each application, follow the recommended dilutions on the product datasheet and optimize empirically for your sample type.

What is the recommended dilution range?

WB: 1:500~1:1000 IHC: 1:50~1:100

Which species does this antibody recognize?

This antibody has been confirmed reactive against: Human,Mouse,Rat. Cross-reactivity with other species may occur due to sequence homology but is not guaranteed without independent validation.

What is the immunogen / antigen used to generate this antibody?

The antiserum was produced against synthesized phosphopeptide derived from human EGFR around the phosphorylation site of tyrosine1092 (P-E-YP-I-N).

How should this antibody be stored and handled?

This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt. Avoid repeated freeze-thaw cycles. Centrifuge vials briefly before opening. Dilute immediately before use.

Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

Try Celltrypse Free – Request Your Sample Today