| Field | Specification |
|---|---|
| Target | |
| Alternative names | EH domain-containing protein 3; PAST homolog 3; EHD3; EHD2; PAST3 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human EHD3, which shares 93.8% and 100% amino acid (aa) sequence identity with mouse and rat EHD3, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects EH domain-containing protein 3 (gene EHD3) in human, monkey, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 60.9 kDa, and the supplier reports an observed band at about 70 kDa.
EHD3 is an EH-domain ATPase that binds and tubulates membranes. It supports transport from early endosomes to the recycling compartment and to the Golgi, and in the heart it helps target ion channels and transporters to the membrane.
| Target | EH domain-containing protein 3 (gene EHD3; UniProt Q9NZN3, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Monkey, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 60.9 kDa |
| Observed MW | 70 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.25 µg/mL (Human, Monkey, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human, Mouse, Rat) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human placenta tissue, human A549 cells, human HeLa cells, human CCRF-CEM cells, human U-87MG cells, human SY-SY5Y cells, monkey heart tissue, rat heart tissue, rat brain tissue, rat kidney tissue, rat liver tissue, mouse heart tissue, mouse brain tissue, mouse kidney tissue, mouse liver tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.25 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse brain tissue, human ovarian serous adenocarcinoma tissue, human renal clear cells carcinoma tissue, human breast cancer tissue, human lung cancer tissue, human rectal cancer tissue, human gastric adenocarcinoma tissue, rat brain tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): MCF-7 cells.
Samples with a confirmed signal (flow cytometry): MCF-7 cells.
Immunogen
Synthetic peptide from the C-terminal region of human EHD3. Sequence identity with the mouse and rat orthologs: 93.8% and 100%, respectively.
Reactivity Notes
The supplier lists reactivity with human, monkey, mouse and rat. UniProt places EH domain-containing protein 3 in the recycling endosome membrane, cell membrane and cell projection. Tissue expression noted by UniProt (human): Highly expressed in heart and brain and moderately expressed in kidney, liver, and placenta. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
