Anti-eIF2B epsilon Rabbit Monoclonal Antibody

Overview

This product is an anti-EIF2B5 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone 18E85; isotype IgG; reactivity: Human. Reported application contexts include WB, IHC, ICC, IF (as provided in the source record). Boster Bio Anti-eIF2B epsilon Rabbit Monoclonal Antibody catalog # M03801. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human.

Key elements and design rationale

• Target: EIF2B5 (Cell division cycle 5-like protein).
• Antibody format: Monoclonal; clone 18E85; isotype IgG.
• Host: Rabbit.
• Species reactivity: Human (confirm in your model system with appropriate controls).

This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.

Biological background

EIF2B5 (protein: T-cell surface glycoprotein CD3 zeta chain) is a commonly studied target in molecular and cellular biology. Functional context (as provided): DNA-binding protein involved in cell cycle control. May act as a transcription activator. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. The PRP19-CDC5L complex may also play a role in the response to DNA damage (DDR). . Reported cellular localization context: Nucleus . Nucleus speckle . Cytoplasm . May shuttle between cytoplasm and nucleus. . Tissue expression notes (as provided): Ubiquitously expressed in both fetal and adult tissues. .

Research relevance and current trends

• Research context keywords from the source record include: DNA/RNA,Epigenetics and Nuclear Signaling,RNA Processing,Splicing.
• Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
• Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.

Common research applications

• Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
• Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
• Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.

Workflow ideas (metafield): Validate EIF2B5 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect EIF2B5 expression by Western blot in cell or tissue lysates, Detect EIF2B5 in FFPE tissue sections by immunohistochemistry, Localize EIF2B5 by immunofluorescence/immunocytochemistry in cultured cells

Notes for experimental interpretation

• Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
• Apparent molecular weight may vary by sample type and processing (observed MW: 80 kDa; calculated MW: 92251 MW).
• Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.

Additional product details (from the source record)

• Molecular weight (observed): 80 kDa
• Cellular localization (provided): Nucleus . Nucleus speckle . Cytoplasm . May shuttle between cytoplasm and nucleus. .
• Tissue details (provided): Ubiquitously expressed in both fetal and adult tissues. .

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.