| Field | Specification |
|---|---|
| Target | |
| Alternative names | Protein argonaute-3 ;Argonaute3 ;hAgo3;Argonaute RISC catalytic component 3;Eukaryotic translation initiation factor 2C 3 ;eIF-2C 3 ;eIF2C 3 ;AGO3;EIF2C3; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human EIF2C3 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone GGF-1 is a rabbit monoclonal antibody that recognizes protein argonaute-3 (gene AGO3) in human, mouse and rat samples and is validated for Western blot and IHC. The predicted molecular weight is 97.4 kDa, and the supplier reports an observed band at about 97 kDa.
Argonaute-3 (AGO3) is an Argonaute family protein that loads microRNAs and represses translation of complementary mRNAs. It shows slicer activity only on selected RNAs and has been linked to small RNA pathways that control gene expression in stem cells.
| Target | Protein argonaute-3 (gene AGO3; UniProt Q9H9G7, human) |
|---|---|
| Clone | GGF-1 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 97.4 kDa |
| Observed MW | 97 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): human brain.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): mouse brain tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide based on the sequence of human EIF2C3.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places protein argonaute-3 in the cytoplasm. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
