| Field | Specification |
|---|---|
| Target | |
| Alternative names | Eukaryotic translation initiation factor 3 subunit B; eIF3b; Eukaryotic translation initiation factor 3 subunit 9; Prt1 homolog; hPrt1; eIF-3-eta; eIF3 p110; eIF3 p116; EIF3S9 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human eIF3B |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 25E26 is a rabbit monoclonal antibody that recognizes eukaryotic translation initiation factor 3 subunit B (gene EIF3B) in human, mouse and rat samples and is validated for Western blot, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 92.5 kDa, and the supplier reports an observed band at about 116 kDa.
EIF3B is a core scaffold subunit of eIF3, the large initiation factor that binds the 40S ribosomal subunit. It helps assemble the pre-initiation complex with eIF1, eIF1A, eIF2 and eIF5 and supports mRNA recruitment during translation initiation.
| Target | Eukaryotic translation initiation factor 3 subunit B (gene EIF3B; UniProt P55884, human) |
|---|---|
| Clone | 25E26 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 92.5 kDa |
| Observed MW | 116 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): A431 cells, 293T cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human eIF3B.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places eukaryotic translation initiation factor 3 subunit B in the cytoplasm. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
