Anti-eIF3B Rabbit Monoclonal Antibody

Overview

This product is an anti-EIF3B antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone 25E26; isotype IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB, ICC, IF, IP, Flow (as provided in the source record). Boster Bio Anti-eIF3B Rabbit Monoclonal Antibody catalog # M04318. Tested in WB, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Key elements and design rationale

• Target: EIF3B (Keratin, type I cytoskeletal 13).
• Antibody format: Monoclonal; clone 25E26; isotype IgG.
• Host: Rabbit.
• Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).

This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.

Biological background

EIF3B (protein: T-cell surface glycoprotein CD3 zeta chain) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Serine/threonine-protein kinase involved in various processes such as neuronal proliferation, differentiation, migration and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress- activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK10/JNK3. In turn, MAPK10/JNK3 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN and ATF2 and thus regulates AP-1 transcriptional activity. Plays regulatory roles in the signaling pathways during neuronal apoptosis. Phosphorylates the neuronal microtubule regulator STMN2. Acts in the regulation of the beta-amyloid precursor protein/APP signaling during neuronal differentiation by phosphorylating APP. Participates also in neurite growth in spiral ganglion neurons. Phosphorylates the CLOCK-ARNTL/BMAL1 heterodimer and plays a role in the photic regulation of the circadian clock (PubMed:22441692). . Reported cellular localization context: Cytoplasm . Membrane ; Lipid-anchor . Nucleus . Mitochondrion . Palmitoylation regulates MAPK10 trafficking to cytoskeleton. Recruited to the mitochondria in the presence of SARM1 (By similarity). . Tissue expression notes (as provided): Expressed in some epidermal sweat gland ducts (at protein level) and in exocervix, esophagus and placenta. .

Research relevance and current trends

• Research context keywords from the source record include: Cancer,Cancer Metabolism,Endocrine Metabolism,Endocrine System,Growth Factors,Growth Factors/Hormones,Hormone Biosynthesis,Metabolic Signaling Pathway,Metabolism,Neuroscience,Pathways and Processes,Signal Transduction.
• Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
• Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.

Common research applications

• Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
• Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
• Flow cytometry: quantify target-positive populations and compare shifts in marker distributions.
• Immunoprecipitation (IP): enrich target complexes for downstream immunoblot or interaction analyses.

Workflow ideas (metafield): Validate EIF3B antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect EIF3B expression by Western blot in cell or tissue lysates, Localize EIF3B by immunofluorescence/immunocytochemistry in cultured cells, Quantify EIF3B-positive cells by flow cytometry in single-cell suspensions, Enrich EIF3B by immunoprecipitation from lysates for downstream analysis

Notes for experimental interpretation

• Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
• Apparent molecular weight may vary by sample type and processing (observed MW: 120 kDa; calculated MW: 49588 MW).
• Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.

Additional product details (from the source record)

• Molecular weight (observed): 120 kDa
• Cellular localization (provided): Cytoplasm . Membrane ; Lipid-anchor . Nucleus . Mitochondrion . Palmitoylation regulates MAPK10 trafficking to cytoskeleton. Recruited to the mitochondria in the presence of SARM1 (By similarity). .
• Tissue details (provided): Expressed in some epidermal sweat gland ducts (at protein level) and in exocervix, esophagus and placenta. .

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.