Anti-EIF3e Antibody Picoband® (monoclonal, 10F5H6)

Overview

Anti-EIF3e Antibody Picoband® (monoclonal, 10F5H6) is an antibody for EIF3E detection raised in Mouse (Monoclonal, clone Clone: 10F5H6, Mouse IgG2b), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.

Key elements and design rationale

• Target: EIF3E (CD5 molecule); UniProt: P60228
• Antibody format: Mouse, Monoclonal, clone Clone: 10F5H6, Mouse IgG2b
• Molecular weight: 52 kDa
• Applications: WB, IHC, Flow Cytometry, ELISA

Vendor description (summary): Boster Bio Anti-EIF3e Antibody Picoband® (monoclonal, 10F5H6) catalog # M00481-1.

Biological background

Biological context: May act as a receptor in regulating T-cell proliferation.

Expression and localization notes: cellular localization: Cell membrane; Single-pass type I membrane protein, tissue context: Brain, liver, placenta, lymphocytes and erythrocytes..

Common research applications

• Western blotting (WB): Compare EIF3E levels across samples and conditions using appropriate loading and biological controls.
• Immunohistochemistry (IHC): Evaluate spatial distribution of EIF3E in tissue sections, considering fixation and antigen retrieval effects.
• Flow cytometry: Quantify EIF3E-positive populations in single-cell suspensions with appropriate gating and controls.
• ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.

Notes for experimental interpretation

• Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
• Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.

Additional product notes (from provided fields)

• Background: Eukaryotic translation initiation factor 3 subunit E is a protein that in humans is encoded by the EIF3E gene. The human homolog of EIF3E is located on chromosome region 8q22-q23. It is composed of 13 exons that span 45 kb of genomic DNA. EIF3E is the component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis ts localization/assembly. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). And the eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
• Cross reactivity: No cross-reactivity with other proteins.
• Cellular localization: Cell membrane; Single-pass type I membrane protein
• Tissue details: Brain, liver, placenta, lymphocytes and erythrocytes.
• Research category: Cell Adhesion,Cell Type Markers,Cytoskeleton/ECM,Hematopoietic Progenitors,Immunology,Integrins,Myeloid,Signal Transduction,Stem Cells

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.