Anti-EIF4B Antibody Picoband®

Overview

Anti-EIF4B Antibody Picoband® is an antibody for EIF4B detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.

Key elements and design rationale

• Target: EIF4B (complement C7); UniProt: P23588
• Antibody format: Rabbit, Polyclonal, Rabbit IgG
• Molecular weight: 80 kDa, calculated 55914 MW
• Applications: WB, IHC, Flow Cytometry, ELISA

Vendor description (summary): Boster Bio Anti-EIF4B Antibody Picoband® catalog # A00846-1.

Biological background

Biological context: Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C7 serves as a membrane anchor.

Expression and localization notes: cellular localization: Secreted., tissue context: Widely expressed..

Common research applications

• Western blotting (WB): Compare EIF4B levels across samples and conditions using appropriate loading and biological controls.
• Immunohistochemistry (IHC): Evaluate spatial distribution of EIF4B in tissue sections, considering fixation and antigen retrieval effects.
• Flow cytometry: Quantify EIF4B-positive populations in single-cell suspensions with appropriate gating and controls.
• ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.

Notes for experimental interpretation

• Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
• Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.

Additional product notes (from provided fields)

• Background: Eukaryotic translation initiation factor 4B is a protein that in humans is encoded by the EIF4B gene. Eukaryotic initiation factor 4B (eIF4B) is thought to assist the eIF4F complex in translation initiation. In plants, eIF4B is known to interact with the poly-(A) binding protein, increasing its poly-(A) binding activity. Heat shock and serum starvation cause dephosphorylation of eIF4B at multiple sites with kinetics similar to those of the corresponding inhibition of translation, while phosphorylation of eIF4B following insulin treatment correlates well with an observed increase in translation. Multiple kinases, including p70 S6 kinase, can phosphorylate eIF4B in vitro, and at least one serum-inducible eIF4B phosphorylation site is sensitive to rapamycin and LY294002. Recently, Ser406 was identified as a novel phosphorylation site regulated by mitogens, and the phosphorylation of this site is dependent on MEK and mTOR activity. This phosphorylation is shown to be essential for the translational activity of eIF4B.
• Cross reactivity: No cross-reactivity with other proteins.
• Cellular localization: Secreted.
• Tissue details: Widely expressed.
• Research category: Angiogenesis,Cancer,Cardiovascular,Developmental Biology,Embryogenesis,Growth Factors,Growth Factors/Hormones,Lineage Specification,Mesenchymal Stem Cells,Signal Transduction,Stem Cells

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.