| Field | Specification |
|---|---|
| Target | |
| Alternative names | Eukaryotic translation initiation factor 4E-binding protein 1;4E-BP1;eIF4E-binding protein 1;Phosphorylated heat- and acid-stable protein regulated by insulin 1;PHAS-I;EIF4EBP1; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human eIF4EBP1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone IGG-5 is a rabbit monoclonal antibody that recognizes eukaryotic translation initiation factor 4E-binding protein 1 (gene EIF4EBP1) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 12.6 kDa, and the supplier reports an observed band at about 18 kDa.
4E-BP1 (EIF4EBP1) is a translational repressor that binds eIF4E and prevents it from joining eIF4G. When mTORC1 phosphorylates 4E-BP1, it releases eIF4E and cap-dependent translation increases, so phospho-4E-BP1 is a standard readout of mTOR activity.
| Target | Eukaryotic translation initiation factor 4E-binding protein 1 (gene EIF4EBP1; UniProt Q13541, human) |
|---|---|
| Clone | IGG-5 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 12.6 kDa |
| Observed MW | 18 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:1000–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): K562 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): human breast carcinoma tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Samples with confirmed staining (ICC/IF): HeLa cells.
Immunogen
Synthetic peptide based on the sequence of human eIF4EBP1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places eukaryotic translation initiation factor 4E-binding protein 1 in the cytoplasm and nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Ruyue Xu et al. (2021) LY‑294002 enhances the chemosensitivity of liver cancer to oxaliplatin by blocking the PI3K/AKT/HIF‑1α pathway. Molecular Medicine Reports. 10.3892/mmr.2021.12147
