| Field | Specification |
|---|---|
| Target | |
| Alternative names | Elafin; Elastase-specific inhibitor; ESI; Peptidase inhibitor 3; PI-3; Protease inhibitor WAP3; Skin-derived antileukoproteinase; SKALP; WAP four-disulfide core domain protein 14; PI3; WAP3; WFDC14 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human Elafin/Skalp recombinant protein (Position: A61-Q117). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone C7 is a mouse monoclonal antibody that recognizes elafin (gene PI3) in human samples and is validated for ELISA and IHC. The predicted molecular weight is 12.3 kDa.
Elafin (PI3, also called SKALP) is a small secreted serine protease inhibitor produced by skin, airway and other epithelia. It blocks neutrophil elastase and proteinase 3, protecting tissues from proteolytic damage during inflammation, and it also has antimicrobial activity, so it is measured in studies of skin disease, lung inflammation and mucosal defense.
| Target | Elafin (gene PI3; UniProt P19957, human) |
|---|---|
| Clone | C7 |
| Host / Clonality / Isotype | Mouse / Monoclonal / Mouse IgG1 |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 12.3 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL |
|---|---|
| ELISA (capture antibody) | 1–5 µg/mL |
Samples with confirmed staining (IHC): human tonsil tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Immunogen
Recombinant human Elafin/Skalp fragment (Ala61–Gln117), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes elafin as a secreted protein.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Jingwen Deng et al. (2023) Multi-omics approach identifies PI3 as a biomarker for disease severity and hyper-keratinization in psoriasis. Journal of Dermatological Science. 10.1016/j.jdermsci.2023.07.005
