| Field | Specification |
|---|---|
| Target | |
| Alternative names | Emerin; EMD; EDMD; STA |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human Emerin, different from the related mouse sequence by eight amino acids, and from the related rat sequence by nine amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 5A10 is a mouse monoclonal antibody that recognizes emerin (gene EMD) in human samples and is validated for flow cytometry, IHC, ICC/IF and Western blot. The predicted molecular weight is 29.0 kDa, and the supplier reports an observed band at about 34 kDa.
Emerin (EMD) is an inner nuclear membrane protein of the LEM-domain family that binds lamins, BAF and nuclear actin. It helps organize the nuclear envelope, links centrosomes to the nucleus and restrains nuclear β-catenin signaling. Loss of emerin causes X-linked Emery-Dreifuss muscular dystrophy, so it is widely studied in nuclear mechanics and laminopathies.
| Target | Emerin (gene EMD; UniProt P50402, human) |
|---|---|
| Clone | 5A10 |
| Host / Clonality / Isotype | Mouse / Monoclonal / Mouse IgG1 |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 29.0 kDa |
| Observed MW | 34 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL |
| IHC (frozen sections) | 0.5–1 µg/mL |
| Immunocytochemistry / Immunofluorescence | 0.5–1 µg/mL |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells |
Samples with a confirmed band (WB): human HeLa cells, human placenta tissue, human Caco-2 cells, human HepG2 cells, Rabbit IgG, Marker 1113, human Jurkat whole cellhuman MDA-MB-453 cells, human SK-OV-3 cells, human SW620 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): human gastric cancer tissue, human rectal cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Samples with a confirmed signal (flow cytometry): A431 cells.
Immunogen
Synthetic peptide from the N-terminal region of human emerin. It differs from the mouse sequence at eight residues and from the rat sequence at nine residues.
Reactivity Notes
The supplier lists reactivity with human. UniProt places emerin in the nucleus inner membrane, nucleoplasmic side and nucleus outer membrane. Tissue expression noted by UniProt (human): Skeletal muscle, heart, colon, testis, ovary and pancreas. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
He Tianzhen et al. (2022) Collective analysis of the expression and prognosis for LEM-domain proteins in prostate cancer. World Journal of Surgical Oncology. 10.1186/s12957-022-02640-z
