| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Echinoderm microtubule-associated protein-like 4; EMAP-4; Restrictedly overexpressed proliferation-associated protein; Ropp 120; EML4; C2orf2; EMAPL4 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E. coli-derived human EML4 recombinant protein (Position: K207-N423). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of EML4 (Insulin-like growth factor-binding protein 1) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-EML4 Antibody Picoband® catalog # A00930-1. Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E. coli-derived human EML4 recombinant protein (Position: K207-N423). (reported region: K207-N423).
- Molecular weight context: reported MW: 120 kDa; calculated MW: 108 kDa
- Reactivity: Human
- Applications: ELISA, Flow Cytometry, IF, IHC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Insulin-like growth factor-binding protein 1; echinoderm microtubule associated protein like 4. Echinoderm microtubule-associated protein-like 4 is a protein that in humans is encoded by the EML4gene. This gene is a member of the echinoderm microtubule associated protein-like family, and it mapped to 2p21. The encoded WD-repeat protein may be involved in microtubule formation. Abnormal fusion of parts of this gene with portions of the anaplastic lymphoma receptor tyrosine kinase gene, which generates EML4-ALK fusion transcripts, is one of the primary mutations associated with non-small cell lung cancer. Alternative splicing of this gene results in two transcript variants. Functional note: May modify the assembly dynamics of microtubules, such that microtubules are slightly longer, but more dynamic. . Reported localization: Cytoplasm, cytoskeleton. Expression/tissue context: High expression in adult thyroid, lower expression in adult and fetal kidney and fetal brain. Not expressed in other tissues.
Research relevance and current trends
- Cytoskeleton: Researchers commonly examine how EML4 (Insulin-like growth factor-binding protein 1) relates to this theme using model systems and orthogonal readouts.
- Microtubules: Researchers commonly examine how EML4 (Insulin-like growth factor-binding protein 1) relates to this theme using model systems and orthogonal readouts.
- Subcellular Markers: Researchers commonly examine how EML4 (Insulin-like growth factor-binding protein 1) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative EML4 (Insulin-like growth factor-binding protein 1) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of EML4 (Insulin-like growth factor-binding protein 1) across tissue regions and cell types using matched controls.
- IF/ICC: evaluate subcellular localization and co-localization patterns; signal can depend on fixation/permeabilization and epitope accessibility.
- Flow cytometry: quantify target-positive populations and shifts in expression; gating strategy and background staining controls are essential.
- ELISA-compatible use: when applicable, interpret signal as relative abundance across sample sets with consistent handling and dilution strategy.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
