| Field | Specification |
|---|---|
| Target | |
| Alternative names | Endothelin-1 receptor; Endothelin receptor type A; ET-A; ETA-R; hET-AR; EDNRA; ETA; ETRA |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human Endothelin A Receptor/ET-A/EDNRA recombinant protein (Position: N32-Q381). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes endothelin-1 receptor (gene EDNRA) in human samples and is validated for Western blot, IHC, flow cytometry and ELISA. The predicted molecular weight is 48.7 kDa, and the supplier reports an observed band at about 50 kDa.
Endothelin receptor type A (EDNRA) is a G protein-coupled receptor that binds endothelin-1 with high preference over other endothelins. On vascular smooth muscle it signals through phospholipase C and Ca2+ to cause contraction, and it is a drug target in pulmonary arterial hypertension research.
| Target | Endothelin-1 receptor (gene EDNRA; UniProt P25101, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.01 mg NaN3 |
| Calculated MW | 48.7 kDa |
| Observed MW | 50 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HEK293 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): human lung cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with a confirmed signal (flow cytometry): A549 cells.
Immunogen
Recombinant human Endothelin A Receptor/ET-A/EDNRA fragment (Asn32–Gln381), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places endothelin-1 receptor in the cell membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Hongyu Su et al. (2024) Endothelin-1 potentiated constriction in preeclampsia placental veins: role of ETAR/ETBR/CaV1.2/CALD1. Placenta. 10.1016/j.placenta.2024.10.011
Hong-Jun Peng et al. (2010) The separate roles of endothelin receptors participate in remodeling of matrix metalloproteinase and connexin 43 of cardiac fibroblasts in maladaptive response to isoproterenol. European Journal of Pharmacology. 10.1016/j.ejphar.2010.02.001
Xu Ming et al. (2008) Normalizing NADPH Oxidase Contributes to Attenuating Diabetic Nephropathy by the Dual Endothelin Receptor Antagonist CPU0213 in Rats. American Journal of Nephrology. 10.1159/000157628
Lu Luo et al. (2008) EFFECT OF THE ENDOTHELIN RECEPTOR ANTAGONIST CPU0213, AND ITS MODULATION BY RIFAMPIN, ON CARDIAC AND VASCULAR TISSUE FOLLOWING CHRONIC ISOPROTERENOL TREATMENT. Clinical and Experimental Pharmacology and Physiology. 10.1111/j.1440-1681.2007.04856.x
