| Field | Specification |
|---|---|
| Target | |
| Alternative names | Ectonucleotide pyrophosphatase/phosphodiesterase family member 1; E-NPP 1; Alkaline phosphodiesterase I; Membrane component chromosome 6 surface marker 1; Nucleotide diphosphatase; Nucleotide pyrophosphatase; NPPase; Phosphodiesterase I/nucleotide pyrophosphatase 1; Plasma-cell membrane glycoprotein PC-1; ENPP1; M6S1; NPPS; PC1; PDNP1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human ENPP1/PC1 recombinant protein (Position: E347-N789). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects ectonucleotide pyrophosphatase/phosphodiesterase family member 1 (gene ENPP1) in human samples and is validated for Western blot, IHC, flow cytometry and ELISA. The predicted molecular weight is 104.9 kDa, and the supplier reports an observed band at about 130–150 kDa.
ENPP1 (also called PC-1) is a type II transmembrane ectoenzyme that hydrolyzes ATP to release inorganic pyrophosphate, a key inhibitor of hydroxyapatite deposition. It therefore helps control bone mineralization and prevents soft tissue calcification. ENPP1 also degrades the immune messenger 2′3′-cGAMP and has been linked to insulin signaling, making it a subject of skeletal, vascular and immunology research.
| Target | Ectonucleotide pyrophosphatase/phosphodiesterase family member 1 (gene ENPP1; UniProt P22413, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 104.9 kDa |
| Observed MW | 130–150 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 1–2 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HepG2 cells, human hccp tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human endometrial cancer tissue, human liver cancer tissue, human placenta tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with a confirmed signal (flow cytometry): HepG2 cells.
Immunogen
Recombinant human ENPP1/PC1 fragment (Glu347–Asn789), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places ectonucleotide pyrophosphatase/phosphodiesterase family member 1 in the basolateral cell membrane. Tissue expression noted by UniProt (human): Expressed in plasma cells and also in a number of non-lymphoid tissues, including the distal convoluted tubule of the kidney, chondrocytes and epididymis. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
