| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Bestrophin-1; TU15B; Vitelliform macular dystrophy protein 2; BEST1; VMD2 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived rat Eotaxin/Ccl11 recombinant protein (Position: H24-P97). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-Eotaxin/Ccl11 Antibody is an antibody for Ccl11 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: Ccl11 (bestrophin 1); UniProt: P97545
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 75 kDa, calculated 51561 MW
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-Eotaxin/Ccl11 Antibody Picoband® catalog # A01438-2.
Biological background
Biological context: Forms calcium-sensitive chloride channels. Highly permeable to bicarbonate.
Expression and localization notes: cellular localization: Cell membrane., tissue context: Predominantly expressed in the basolateral membrane of the retinal pigment epithelium..
Common research applications
- Western blotting (WB): Compare Ccl11 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of Ccl11 in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify Ccl11-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: CCL11 (Chemokine (C-C motif) ligand 11), also known as Eotaxin, is a small cytokine belonging to the CC chemokine family. Eotaxin selectively recruits eosinophils by inducing their chemotaxis, and therefore, is implicated in allergic responses. The effects of Eotaxin are mediated by its binding to a G-protein-linked receptor known as a chemokine receptor. Chemokine receptors for which Eotaxin is a ligand include CCR2, CCR3 and CCR5. However, it has been found that eotaxin-1 (CCL11) has high degree selectivity for its receptor, such that they are inactive on neutrophils and monocytes, which do not express CCR3. The gene for human Eotaxin is encoded on three exons and is located on chromosome 17q12.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Cell membrane.
- Tissue details: Predominantly expressed in the basolateral membrane of the retinal pigment epithelium.
- Research category: Cell Adhesion Proteins,ECM Proteins,Neuroscience,Sensory System,Visual System
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
