| Field | Specification |
|---|---|
| Target | |
| Alternative names | Eotaxin; C-C motif chemokine 11; Eosinophil chemotactic protein; Small-inducible cytokine A11; Ccl11; Scya11 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived rat Eotaxin/Ccl11 recombinant protein (Position: H24-P97). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets eotaxin (gene Ccl11) in rat samples and is validated for IHC and ELISA. The predicted molecular weight is 10.9 kDa.
Eotaxin (CCL11) is a C-C chemokine that recruits eosinophils and other leukocytes, mainly by binding the receptor CCR3. It is a key mediator of eosinophil accumulation in allergic inflammation, including asthma and atopic disease, and it has also been studied as a factor whose circulating levels rise with age.
| Target | Eotaxin (gene Ccl11; UniProt P97545, rat) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 10.9 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 2–5 µg/mL (Rat) |
|---|---|
| ELISA | 0.1–0.5 µg/mL (Rat) |
Samples with confirmed staining (IHC): rat lymphaden tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant rat Eotaxin/Ccl11 fragment (His24–Pro97), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with rat. UniProt describes eotaxin as a secreted protein.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
