| Field | Specification |
|---|---|
| Target | |
| Alternative names | Eotaxin;C-C motif chemokine 11;Eosinophil chemotactic protein;Small-inducible cytokine A11;CCL11;SCYA11; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human Eotaxin recombinant protein (Position: G24-P97). Human Eotaxin shares 61.6% and 63% amino acid (aa) sequence identity with mouse and rat Eotaxin, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes eotaxin (gene CCL11) in human, mouse and rat samples and is validated for ELISA, IHC and Western blot. The predicted molecular weight is 10.7 kDa; the supplier reports observed bands at 11, 15 kDa.
Eotaxin (CCL11) is a C-C chemokine that recruits eosinophils and other leukocytes, mainly by binding the receptor CCR3. It is a key mediator of eosinophil accumulation in allergic inflammation, including asthma and atopic disease, and it has also been studied as a factor whose circulating levels rise with age.
| Target | Eotaxin (gene CCL11; UniProt P51671, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.01 mg NaN3; plus stabilizers |
| Calculated MW | 10.7 kDa |
| Observed MW | 11, 15 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): recombinant human Eotaxin/CCL11 protein, recombinant mouse Eotaxin/CCL11 protein, ecombinant rat Eotaxin/CCL11 protein.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 12% SDS-PAGE, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human colon cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human Eotaxin fragment (Gly24–Pro97), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 61.6% and 63%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes eotaxin as a secreted protein. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Guimin Zhang et al. (2012) Distribution Change of Mast Cells in Human Nasal Polyps. Anatomical Record-Advances in Integrative Anatomy and Evolutionary Biology. 10.1002/ar.22430
