| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Eotaxin;C-C motif chemokine 11;Eosinophil chemotactic protein;Small-inducible cytokine A11;CCL11;SCYA11; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E. coli-derived human Eotaxin recombinant protein (Position: G24-P97). Human Eotaxin shares 61.6% and 63% amino acid (aa) sequence identity with mouse and rat Eotaxin, respectively. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of CCL11 (Eotaxin) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-Eotaxin/CCL11 Antibody Picoband® catalog # PB9569. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E. coli-derived human Eotaxin recombinant protein (Position: G24-P97). Human Eotaxin shares 61.6% and 63% amino acid (aa) sequence identity with mouse and rat Eotaxin, respectively. (reported region: G24-P97).
- Molecular weight context: reported MW: 11, 15 kDa; calculated MW: 10732 MW
- Reactivity: Human,Mouse,Rat
- Applications: ELISA, IHC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Eotaxin; Eotaxin. CCL11 (Chemokine (C-C motif) ligand 11), also known as Eotaxin, is a small cytokine belonging to the CC chemokine family. Eotaxin selectively recruits eosinophils by inducing their chemotaxis, and therefore, is implicated in allergic responses. The effects of Eotaxin are mediated by its binding to a G-protein-linked receptor known as a chemokine receptor. Chemokine receptors for which Eotaxin is a ligand include CCR2, CCR3 and CCR5. However, it has been found that eotaxin-1 (CCL11) has high degree selectivity for its receptor, such that they are inactive on neutrophils and monocytes, which do not express CCR3. The gene for human Eotaxin is encoded on three exons and is located on chromosome 17q12. Functional note: In response to the presence of allergens, this protein ly promotes the accumulation of eosinophils, a prominent feature of allergic inflammatory reactions. Binds to CCR3. Reported localization: Secreted. Expression/tissue context: Synthesized in liver and selectively concentrated in bone matrix. Secreted in plasma. It is also found in dentin in much higher quantities than other plasma proteins.
Research relevance and current trends
- Chemokines: Researchers commonly examine how CCL11 (Eotaxin) relates to this theme using model systems and orthogonal readouts.
- Immunology: Researchers commonly examine how CCL11 (Eotaxin) relates to this theme using model systems and orthogonal readouts.
- Innate Immunity: Researchers commonly examine how CCL11 (Eotaxin) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative CCL11 (Eotaxin) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of CCL11 (Eotaxin) across tissue regions and cell types using matched controls.
- ELISA-compatible use: when applicable, interpret signal as relative abundance across sample sets with consistent handling and dilution strategy.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Family / similarity context: Belongs to the intercrine beta (chemokine CC) family.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
