| Field | Specification |
|---|---|
| Target | |
| Alternative names | Band 4.1-like protein 1;Neuronal protein 4.1;4.1N;EPB41L1;KIAA0338; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human EPB41L1 recombinant protein (Position: Q691-S881). Human EPB41L1 shares 91% amino acid (aa) sequence identity with both mouse and rat EPB41L1. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes band 4.1-like protein 1 (gene EPB41L1) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 98.5 kDa, and the supplier reports an observed band at about 100–110 kDa.
Band 4.1-like protein 1 (EPB41L1, also called 4.1N) belongs to the protein 4.1 family of membrane-cytoskeleton linkers. It is enriched in neurons, where it connects the spectrin-actin network with membrane channels, receptors and scaffolding proteins, helping stabilize and shape neuronal membranes.
| Target | Band 4.1-like protein 1 (gene EPB41L1; UniProt Q9H4G0, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 98.5 kDa |
| Observed MW | 100–110 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|
Samples with a confirmed band (WB): human RT4 cells, human Caco-2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human EPB41L1 fragment (Gln691–Ser881), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 91%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places band 4.1-like protein 1 in the cytoplasm. Tissue expression noted by UniProt (human): Highest expression in brain, lower in heart, kidney, pancreas, placenta, lung and skeletal muscle. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
