| Field | Specification |
|---|---|
| Target | |
| Alternative names | Endothelial protein C receptor;Activated protein C receptor;APC receptor;Endothelial cell protein C receptor;CD201;PROCR;EPCR; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human EPCR/CD201 recombinant protein (Position: S18-S210). Human EPCR/CD201 shares 65.5% and 65.6% amino acid (aa) sequence identity with mouse and rat EPCR/CD201, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes endothelial protein C receptor (gene PROCR) in human samples and is validated for ELISA and Western blot. The predicted molecular weight is 26.7 kDa; the supplier reports observed bands at 46 kDa, 52 kDa.
The endothelial protein C receptor (EPCR, PROCR) is a transmembrane protein on endothelial cells that binds protein C and activated protein C. By presenting protein C to the thrombin-thrombomodulin complex it speeds its conversion to the active anticoagulant, and it supports cytoprotective PAR1 signaling; a soluble form circulates in plasma.
| Target | Endothelial protein C receptor (gene PROCR; UniProt Q9UNN8, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 26.7 kDa |
| Observed MW | 46 kDa, 52 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| ELISA | 0.1–0.5 µg/mL |
|---|---|
| Western blot | 0.1–0.5 µg/mL (Human) |
Samples with a confirmed band (WB): A549 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human EPCR/CD201 fragment (Ser18–Ser210), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 65.5% and 65.6%, respectively.
Reactivity Notes
The supplier lists reactivity with human. UniProt places endothelial protein C receptor in the membrane. Tissue expression noted by UniProt (human): Expressed strongly in the endothelial cells of arteries and veins in heart and lung, less intensely in capillaries in the lung and skin, and not at all in the endothelium of small vessels of the liver and kidney. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
