Anti-Eph receptor A3/EPHA3 Antibody Picoband®

Overview

Anti-Eph receptor A3/EPHA3 Antibody Picoband® is an antibody reagent for detection of EPHA3 (tumor necrosis factor receptor superfamily, member 13c). Researchers commonly use anti-EPHA3 antibodies to measure relative expression and localization across biological samples, with assay selection guided by the listed applications (WB, IHC, IF, ICC, Flow, ELISA).

Boster Bio Anti-Eph receptor A3/EPHA3 Antibody Picoband® catalog # A02872-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Key elements and design rationale

• Target: EPHA3 (tumor necrosis factor receptor superfamily, member 13c). Alternative names: Tumor necrosis factor receptor superfamily member 13C; B-cell maturation defect; B-cell-activating factor receptor; BAFF receptor; BAFF-R; BLyS receptor 3; CD268; Tnfrsf13c; Baffr; Bcmd; Br3
• Antibody format: Polyclonal; Rabbit IgG
• Species context: Host: Rabbit, Reactivity: Human
• Purification: Immunogen affinity purified.
• Immunogen: E.coli-derived human Eph receptor A3/EPHA3 recombinant protein (Position: D59-A514).
• Molecular weight context: observed 135 kDa (reported)
• Provided application(s): WB, IHC, IF, ICC, Flow, ELISA

These attributes help contextualize how the antibody is commonly selected (host/clonality/isotype/label) and how signals are interpreted across sample types and assay formats.

Biological background

Function: B-cell receptor specific for TNFSF13B/TALL1/BAFF/BLyS. Promotes the survival of mature B-cells and the B-cell response.

Cellular localization: Membrane; Single-pass type III membrane protein.

Tissue details: Highly expressed in spleen and testis; detected at lower levels in lung and thymus.

Background: Ephrin Receptor EphA3, is also known as human embryo kinase(HEK) or Eph-like tyrosine kinase1(ETK1). Kinases that phosphorylate proteins on tyrosine residues(protein tyrosine kinases; PTKs), such as EPHA3, form a structurally related group of molecules that exhibit functional diversity. Genetic alterations that lead to the inappropriate activation or expression of PTKs may be oncogenic. Many growth factor receptors are PTKs, e.g., the receptors for epidermal growth factor(EGFR), platelet-derived growth factor(PDGFR1;PDGFR2), colony-stimulating factor-1(CSF1R), and stem cell growth factor. The EPHA3 gene is mapped to 3p11.2. Sequence comparison with other PTKs showed a high degree of homology with members of the EPH and ELK(EPHB1) families of receptor tyrosine kinases. Within axial nerves, establishment of discrete afferent and efferent pathways depends on coordinate signaling between coextending sensory and motor projections.

Cross reactivity: No cross-reactivity with other proteins.

Research relevance and current trends

• Quantitative and spatial profiling: expression patterns are increasingly studied across cell states using multiplex imaging and omics-informed validation.
• Isoforms and post-translational modifications: researchers often evaluate how isoform composition and PTMs can shift apparent molecular weight or localization.
• Context-aware interpretation: comparative studies commonly include perturbations (stimulation, inhibition, genetic models) to relate target changes to pathway behavior.

Common research applications

• Western blot (WB): compare relative target abundance and apparent size shifts (e.g., isoforms/PTMs) across conditions.
• Immunohistochemistry (IHC): assess distribution across tissue compartments and compare staining patterns between groups.
• Immunofluorescence / ICC: evaluate subcellular localization and co-localization with compartment markers.
• Flow cytometry: quantify target-positive populations and compare shifts after stimulation or differentiation.

Across these uses, researchers typically interpret changes in signal as relative differences between matched sample groups, considering sample preparation and biological context.

Notes for experimental interpretation

• Apparent molecular weight can vary due to isoforms, proteolysis, glycosylation, phosphorylation, and sample preparation differences.
• Species reactivity and epitope conservation can influence observed signal patterns, especially in cross-species studies.
• Control concepts: include appropriate negative controls (e.g., isotype controls where relevant) and, when feasible, genetic or orthogonal controls (KO/KD, peptide competition, or independent assays) to support interpretation.

For antibody reagents, monoclonal antibodies are often chosen for epitope consistency across lots, while polyclonals may recognize multiple epitopes and can show different background characteristics depending on context.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.