| Field | Specification |
|---|---|
| Target | |
| Alternative names | Ephrin type-B receptor 1; ELK; EPH tyrosine kinase 2; EPH-like kinase 6; EK6; hEK6; Neuronally-expressed EPH-related tyrosine kinase; NET; Tyrosine-protein kinase receptor EPH-2; EPHB1; EPHT2 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Eph receptor B1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 20E05 is a rabbit monoclonal antibody that recognizes ephrin type-B receptor 1 (gene EPHB1) in human, mouse and rat samples and is validated for Western blot and immunoprecipitation. The predicted molecular weight is 109.9 kDa, and the supplier reports an observed band at about 120 kDa.
EphB1 is an Eph-family receptor tyrosine kinase that binds transmembrane ephrin-B ligands on adjacent cells, producing bidirectional signaling. It helps guide axons and neuronal migration, shapes synaptic function in the nervous system and is studied in pain signaling and cancer.
| Target | Ephrin type-B receptor 1 (gene EPHB1; UniProt P54762, human) |
|---|---|
| Clone | 20E05 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 109.9 kDa |
| Observed MW | 120 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunoprecipitation | 1:50 |
Samples with a confirmed band (WB): U87MG cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human eph receptor B1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places ephrin type-B receptor 1 in the cell membrane, early endosome membrane and cell projection. Tissue expression noted by UniProt (human): Preferentially expressed in brain. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
