| Field | Specification |
|---|---|
| Target | |
| Alternative names | Endoplasmic reticulum aminopeptidase 1; ARTS-1; Adipocyte-derived leucine aminopeptidase; A-LAP; Aminopeptidase PILS; Puromycin-insensitive leucyl-specific aminopeptidase; PILS-AP; Type 1 tumor necrosis factor receptor shedding aminopeptidase regulator; ERAP1; APPILS; ARTS1; KIAA0525 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human ERAP1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 24E72 is a rabbit monoclonal antibody that recognizes endoplasmic reticulum aminopeptidase 1 (gene ERAP1) in human, mouse and rat samples and is validated for Western blot and IHC. The predicted molecular weight is 107.2 kDa, and the supplier reports an observed band at about 107 kDa.
ERAP1 is an endoplasmic reticulum aminopeptidase that trims peptides from the N-terminus to the right length for loading onto MHC class I molecules. It thereby shapes the peptide repertoire presented to CD8+ T cells, and ERAP1 variants are associated with ankylosing spondylitis and other MHC class I-linked inflammatory diseases.
| Target | Endoplasmic reticulum aminopeptidase 1 (gene ERAP1; UniProt Q9NZ08, human) |
|---|---|
| Clone | 24E72 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 107.2 kDa |
| Observed MW | 107 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): K562 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human ERAP1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places endoplasmic reticulum aminopeptidase 1 in the endoplasmic reticulum membrane. Tissue expression noted by UniProt (human): Ubiquitous. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
