| Field | Specification |
|---|---|
| Target | |
| Alternative names | Receptor tyrosine-protein kinase erbB-3;2.7.10.1;Proto-oncogene-like protein c-ErbB-3;Tyrosine kinase-type cell surface receptor HER3;ERBB3;HER3; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human ErbB 3. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes receptor tyrosine-protein kinase erbB-3 (gene ERBB3) in human samples and is validated for Western blot. The predicted molecular weight is 148.1 kDa, and the supplier reports an observed band at about 180 kDa.
HER3 (ERBB3) is an EGFR-family receptor for neuregulins with weak intrinsic kinase activity. It signals mainly as a heterodimer with HER2, which phosphorylates HER3 and creates docking sites for PI3K, making HER3 a strong activator of AKT signaling. It is studied in development and in resistance to targeted cancer therapies.
| Target | Receptor tyrosine-protein kinase erbB-3 (gene ERBB3; UniProt P21860, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 148.1 kDa |
| Observed MW | 180 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|
Samples with a confirmed band (WB): human MCF-7 cells, human MDA-MB453 cells, human 293T cells, human T-47D cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 8% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide from the N-terminal region of human erbB 3.
Reactivity Notes
The supplier lists reactivity with human. Tissue expression noted by UniProt (human): Epithelial tissues and brain. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
