| Field | Specification |
|---|---|
| Target | |
| Alternative names | DNA excision repair protein ERCC-1;ERCC1; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human ERCC1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AOFA-5 is a rabbit monoclonal antibody that recognizes DNA excision repair protein ERCC-1 (gene ERCC1) in human and mouse samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 32.6 kDa, and the supplier reports an observed band at about 36 kDa.
ERCC1 forms a heterodimer with XPF (ERCC4), creating a structure-specific endonuclease that makes the 5′ incision in nucleotide excision repair. The ERCC1-XPF complex also acts in interstrand crosslink repair and some recombination pathways, and ERCC1 levels are studied in relation to responses to platinum-based chemotherapy.
| Target | DNA excision repair protein ERCC-1 (gene ERCC1; UniProt P07992, human) |
|---|---|
| Clone | AOFA-5 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 32.6 kDa |
| Observed MW | 36 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human ERCC1.
Reactivity Notes
The supplier lists reactivity with human and mouse. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
