| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | AW546468 antibody; C80305 antibody; MGC18200 antibody; MGC4569 antibody; MGC54054 antibody; OTTHUMP00000147628 antibody; OTTHUMP00000229864 antibody; OTTHUMP00000229865 antibody; OTTHUMP00000229866 antibody; OTTHUMP00000229870 antibody; OTTHUMP00000229871 antibody; OTTHUMP00000229872 antibody; Placental ribonuclease inhibitor antibody; Placental RNase inhibitor antibody; PRI antibody; RAI antibody; RI antibody; Ribonuclease inhibitor antibody; Ribonuclease/angiogenin inhibitor 1 antibody; Ribonuclease/angiogenin inhibitor antibody; RINI_HUMAN antibody; RNase inhibitor antibody; RNH 1 antibody; RNH antibody; RNH1 antibody; Rnh1 ribonuclease/angiogenin inhibitor 1 antibody |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | E.coli-derived human eRF1/ETF1 recombinant protein (Position: D9-K342). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-eRF1/ETF1 Antibody Picoband® (monoclonal, 3E5) is an antibody for ETF1 detection raised in Mouse (Monoclonal, clone Clone: 3 E5, Mouse IgG2b), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, IF, ICC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: ETF1 (ribonuclease/angiogenin inhibitor 1); UniProt: P62495; NCBI Gene: 2107
- Antibody format: Mouse, Monoclonal, clone Clone: 3 E5, Mouse IgG2b
- Molecular weight: 49 kDa
- Applications: WB, IHC, IF, ICC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-eRF1/ETF1 Antibody Picoband® (monoclonal, 3E5) catalog # M04157-1.
Biological background
Biological context: Ribonuclease inhibitor which inhibits RNASE1, RNASE2 and ANG. May play a role in redox homeostasis.
Expression and localization notes: cellular localization: Cytoplasm..
Common research applications
- Western blotting (WB): Compare ETF1 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of ETF1 in tissue sections, considering fixation and antigen retrieval effects.
- Immunofluorescence / ICC: Assess subcellular localization patterns and co-localization with compartment markers in cultured cells.
- Flow cytometry: Quantify ETF1-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: Eukaryotic translation termination factor 1 (eRF1), also known asTB3-1, is a protein that in humans is encoded by the ETF1 gene. It is mapped to 5q31.2. This gene encodes a class-1 polypeptide chain release factor. The encoded protein plays an essential role in ing termination of mRNA translation from the termination codons UAA, UAG and UGA. This protein is a component of the SURF complex which promotes degradation of prematurely terminated mRNAs via the mechanism of nonsense-mediated mRNA decay (NMD). Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 6, 7, and X.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Cytoplasm.
- Research category: Cell Adhesion Proteins,Epigenetics and Nuclear Signaling,Integrins,Mediator Complex,Neuroscience,Receptors,Transcription
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
