| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Keratin, type II cytoskeletal 8; Cytokeratin-8; CK-8; K8; Type-II keratin Kb8; KRT8; CYK8 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human ERp57, different from the related mouse and rat sequences by two amino acids. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-ERp57 Antibody Picoband® (monoclonal, 7E5) is an antibody for PDIA3 detection raised in Mouse (Monoclonal, clone Clone: 7E5, Mouse IgG1), with reported reactivity: Human. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: PDIA3 (keratin 8); UniProt: P30101
- Antibody format: Mouse, Monoclonal, clone Clone: 7E5, Mouse IgG1
- Molecular weight: 57 kDa
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-ERp57 Antibody Picoband® (monoclonal, 7E5) catalog # M01464-4.
Biological background
Biological context: Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
Expression and localization notes: cellular localization: Nucleoplasm. Nucleus matrix. Cytoplasm., tissue context: Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity..
Common research applications
- Western blotting (WB): Compare PDIA3 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of PDIA3 in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify PDIA3-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: PDIA3 (Protein disulfide isomerase family A, member 3), also called GRP58, Erp57 or ER60, is an isomerase enzyme. It is mapped on 15q15.3. PDIA3 is also part of the major histocompatibility complex (MHC) class I peptide-loading complex, which is essential for formation of the final antigen conformation and export from the endoplasmic reticulum to the cell surface. This gene encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Nucleoplasm. Nucleus matrix. Cytoplasm.
- Tissue details: Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.
- Research category: Class I,Cytoskeleton,Cytoskeleton/ECM,Intermediate Filaments,Keratin,Signal Transduction
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
